CoraLite® Plus 488-conjugated Ki-67 Polyclonal antibody

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  IF Staining of HeLa using CL488-27309

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 KI67 antibody (CL488-27309) at dilution of 1:200.

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  FC experiment of HeLa using CL488-27309

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human KI67 (CL488-27309) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of Ramos using CL488-27309

  1x10^6 Ramos cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human KI67 (CL488-27309)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• Featured Product
• KD/KO Validated

Ki-67 Polyclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-27309

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

MKI67, KI67, Proliferation marker protein Ki-67, Ki 67, Antigen KI-67

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: Ramos cells, HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

CL488-27309 targets Ki-67 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Ki-67 fusion protein Ag26266
• Full Name: antigen identified by monoclonal antibody Ki-67
• Calculated Molecular Weight: 359 kDa
• GenBank Accession Number: NM_002417
• Gene Symbol: KI67
• Gene ID (NCBI): 4288
• RRID: AB_2919213
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

Publications

Species	Application	Title
human	IF	J Biochem Mol Toxicol; LncRNA LINC01703 promotes the proliferation, migration, and invasion of colorectal cancer by activating PI3K/AKT pathway; Authors - Yuanhui Xu
human	IF	World J Gastrointest Oncol; Bidirectional effects of the tryptophan metabolite indole-3-acetaldehyde on colorectal cancer; Authors - Ze Dai