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CoraLite® Plus 488-conjugated KI67 Polyclonal antibody
KI67 Polyclonal Antibody for FC (Intra), IF
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-27309
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HeLa cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of CL488-27309 is KI67 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | KI67 fusion protein Ag26266 |
Full Name | antigen identified by monoclonal antibody Ki-67 |
Calculated molecular weight | 359 kDa |
GenBank accession number | NM_002417 |
Gene symbol | MKI67 |
Gene ID (NCBI) | 4288 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 KI67 antibody CL488-27309 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |