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Cytokeratin 14 Polyclonal antibody
Cytokeratin 14 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10143-1-AP
Validation Data Gallery
|Positive WB detected in||A431 cells, rat skin tissue|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human lung cancer tissue, human breast cancer tissue, human cervical cancer tissue, human oesophagus cancer tissue, human skin tissue, human skin cancer tissue, mouse skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, HeLa cells, mouse olfactory epithelium tissue|
|Positive FC detected in||A431 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
10143-1-AP targets Cytokeratin 14 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cytokeratin 14 fusion protein Ag0188|
|Full Name||keratin 14|
|Calculated molecular weight||472 aa, 52 kDa|
|Observed molecular weight||47-50 kDa|
|GenBank accession number||BC002690|
|Gene ID (NCBI)||3861|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.
|Product Specific Protocols|
|WB protocol for Cytokeratin 14 antibody 10143-1-AP||Download protocol|
|IHC protocol for Cytokeratin 14 antibody 10143-1-AP||Download protocol|
|IF protocol for Cytokeratin 14 antibody 10143-1-AP||Download protocol|
|IP protocol for Cytokeratin 14 antibody 10143-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cytokeratin expression in epidermal stem cells in skin adnexal tumors.
Stem Cells Dev
Rapid Differentiation of Multi-Zone Ocular Cells from Human Induced Pluripotent Stem Cells and Generation of Corneal Epithelial and Endothelial Cells.
Eur Surg Res
A novel combination of keratinocyte and autologous microskin grafting to repair full thickness skin loss
J Diabetes Res
Type 2 Diabetic Mellitus Inhibits Skin Renewal through Inhibiting WNT-Dependent Lgr5+ Hair Follicle Stem Cell Activation in C57BL/6 Mice.
Adv Healthc Mater
Bioglass Activated Albumin Hydrogels for Wound Healing.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Parmveer (Verified Customer) (10-20-2019)
Worked well to detect basal cells around the prostate gland of formalin fixed tissue
Joshua (Verified Customer) (05-10-2019)
Human Keratinocytes fixed in 4% paraformaldehyde and stained for KRT14. Goat anti-rabbit 488. Bright, specific staining observed.