|Positive WB detected in||A431 cells, rat skin tissue|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human lung cancer tissue, human breast cancer tissue, human cervical cancer tissue, human oesophagus cancer tissue, human skin tissue, human skin cancer tissue, mouse skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, mouse olfactory epithelium tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10143-1-AP targets Cytokeratin 14 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cytokeratin 14 fusion protein Ag0188|
|Full Name||keratin 14|
|Calculated molecular weight||472 aa, 52 kDa|
|Observed molecular weight||47-50 kDa|
|GenBank accession number||BC002690|
|Gene ID (NCBI)||3861|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.
|Product Specific Protocols|
|WB protocol for Cytokeratin 14 antibody 10143-1-AP||Download protocol|
|IHC protocol for Cytokeratin 14 antibody 10143-1-AP||Download protocol|
|IF protocol for Cytokeratin 14 antibody 10143-1-AP||Download protocol|
|IP protocol for Cytokeratin 14 antibody 10143-1-AP||Download protocol|
|FC protocol for Cytokeratin 14 antibody 10143-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Parmveer (Verified Customer) (10-20-2019)
Worked well to detect basal cells around the prostate gland of formalin fixed tissue
Joshua (Verified Customer) (05-10-2019)
Human Keratinocytes fixed in 4% paraformaldehyde and stained for KRT14. Goat anti-rabbit 488. Bright, specific staining observed.