CoraLite®594-conjugated Cytokeratin 14 Polyclonal antibody

Cytokeratin 14 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10143

Synonyms

CK 14, CK14, Cytokeratin 14, EBS3, EBS4, K14, keratin 14, KRT14, NFJ



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 10143-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-10143 targets Cytokeratin 14 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 14 fusion protein Ag0188
Full Name keratin 14
Calculated molecular weight 472 aa, 52 kDa
GenBank accession numberBC002690
Gene symbol KRT14
Gene ID (NCBI) 3861
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.

Protocols

Product Specific Protocols
IF protocol for CL594 Cytokeratin 14 antibody CL594-10143Download protocol
Standard Protocols
Click here to view our Standard Protocols