MAGOH Monoclonal antibody

MAGOH Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, MOUSE, RAT, RABBIT

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G6B9

Cat no : 68293-1-Ig

Synonyms

MAGOH, MAGOHA, Protein mago nashi homolog



Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

68293-1-Ig targets MAGOH in WB, ELISA applications and shows reactivity with Human, MOUSE, RAT, RABBIT samples.

Tested Reactivity Human, MOUSE, RAT, RABBIT
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAGOH fusion protein Ag3004
Full Name mago-nashi homolog, proliferation-associated (Drosophila)
Calculated molecular weight 146 aa, 17 kDa
Observed molecular weight17 kDa
GenBank accession numberBC018211
Gene symbol MAGOH
Gene ID (NCBI) 4116
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGOH, belonging to the mago nashi family, is a component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). MAGOH regulates the transcriptional activation of STAT3 by interfering complex formation between STAT3 and a core EJC component Y14.

Protocols

Product Specific Protocols
WB protocol for MAGOH antibody 68293-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols