MBNL1 Monoclonal antibody

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  WB analysis using 66837-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66837-1-Ig (MBNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 66837-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66837-1-Ig (MBNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66837-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66837-1-Ig (MBNL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66837-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66837-1-Ig (MBNL1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 66837-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MBNL1 antibody (66837-1-Ig, Clone: 1H2C12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

MBNL1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66837-1-Ig

Clone No.1H2C12

Publications(6)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

1H2C12, EXP, EXP35, EXP40, EXP42

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Tested Applications

• Positive WB detected in: HeLa cells, rat heart tissue, Jurkat cells, HEK-293 cells, C2C12 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

66837-1-Ig targets MBNL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7752

  Product name: Recombinant human MBNL1 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-343 aa of BC050535

  Sequence: MGRCSRENCKYLHPPPHLKTQLEINGRNNLIQQKNMAMLAQQMQLANAMMPGAPLQPVPMFSVAPSLATNASAAAFNPYLGPVSPSLVPAEILPTAPMLVTGNPGVPVPAAAAAAAQKLMRTDRLEVCREYQRGNCNRGENDCRFAHPADSTMIDTNDNTVTVCMDYIKGRCSREKCKYFHPPAHLQAKIKAAQYQVNQAAAAQAAATAAAMTQSAVKSLKRPLEATFDLGIPQAVLPPLPKRPALEKTNGATAVFNTGIFQYQQALANMQLQQHTAFLPPGSILCMTPATSVVPMVHGATPATVSAATTSATSVPFAATATANQIPIISAEHLTSHKYVTQM

• Full Name: muscleblind-like (Drosophila)
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC050535
• Gene Symbol: MBNL1
• Gene ID (NCBI): 4154
• RRID: AB_2882180
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9NR56
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MBNL1 (muscleblind-like 1) is one of the MBNL proteins containing 3 members (MBNL1-3) in vertebrates. And MBNL1 is widely expressed in adult tissues including brain, heart and skeletal muscle (PMID:25274774). As a highly conserved RNA-binding protein, MBNL1 regulates alternative splicing, alternative polyadenylation, mRNA stability and mRNA localization in the nucleus. The neurological diseases DM1 and DM2 (myotonic dystrophy type 1 and type2) are caused by expansion of repetitive sequences in non-coding regions in the DMPK gene because of the inappropriate patterns of alternative splicing owing to MBNL1(PMID:24354850). The antibody could recognize the common full-length protein in applications.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nucleic Acids Res; MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation; Authors - Bangming Jin
human	WB	Front Immunol; A comprehensive cuproptosis score and associated gene signatures reveal prognostic and immunological features of idiopathic pulmonary fibrosis; Authors - Chuanqing Jing
human	IF	Int J Nanomedicine; Exosome-Delivered circSTAU2 Inhibits the Progression of Gastric Cancer by Targeting the miR-589/CAPZA1 Axis; Authors - Chenggang Zhang
human	WB,IP,IF	Genes (Basel); Comparative Analysis of MBNL1 Antibodies: Characterization of Recognition Sites and Detection of RNA Foci Colocalization; Authors - Yoshitaka Aoki
human	WB,IP,IHC,IF	Cell Death Dis; The MBNL1/circNTRK2/PAX5 pathway regulates aerobic glycolysis in glioblastoma cells by encoding a novel protein NTRK2-243aa; Authors - Yubo Zhao ; KD Validated
human	WB	Cancer Biomark; Systematic exploration of prognostic alternative splicing events related to tumor immune microenvironment of Clear Cell Renal Cell Carcinoma; Authors - Hongwei Wu