MCM5 Polyclonal antibody, PBS Only

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  WB analysis using 11703-1-AP (same clone as 11703-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11703-1-AP (MCM5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11703-1-AP (same clone as 11703-1-PBS)

  WB result of MCM5 antibody (11703-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM5 transfected HeLa cells. This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11703-1-AP (same clone as 11703-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11703-1-AP (MCM5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 11703-1-AP (same clone as 11703-1-PBS)

  IP result of anti-MCM5 (IP:11703-1-AP, 4ug; Detection:11703-1-AP 1:4000) with Jurkat cells lysate 2400 ug. This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 11703-1-AP (same clone as 11703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse thymus using 11703-1-AP (same clone as 11703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 11703-1-AP (same clone as 11703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 11703-1-AP (same clone as 11703-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11703-1-AP (same clone as 11703-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM5 antibody (11703-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11703-1-PBS in a different storage buffer formulation.

MCM5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 11703-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CDC46, EC:3.6.4.12, P1 CDC46, P1-CDC46

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Product Information

11703-1-PBS targets MCM5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2271

  Product name: Recombinant human MCM5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 388-734 aa of BC000142

  Sequence: SQLLKFVEKCSPIGVYTSGKGSSAAGLTASVMRDPSSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSVFGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLHVSALTQTQAVEGEIDLAKLKKFIAYCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDSDRRSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALRLFQVSTLDAALSGTLSGVEGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTKQKYPEHAIHKVLQLMLRRGEIQHRMQRKVLYRLK

• Full Name: minichromosome maintenance complex component 5
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 82-88 kDa
• GenBank Accession Number: BC000142
• Gene Symbol: MCM5
• Gene ID (NCBI): 4174
• RRID: AB_2235162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P33992
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Minichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.