MCM5 Polyclonal antibody

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  WB analysis using 11703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11703-1-AP (MCM5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11703-1-AP (MCM5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 11703-1-AP

  IP result of anti-MCM5 (IP:11703-1-AP, 4ug; Detection:11703-1-AP 1:4000) with Jurkat cells lysate 2400 ug.

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  IHC staining of mouse thymus using 11703-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse thymus using 11703-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 11703-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 11703-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11703-1-AP (MCM5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11703-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM5 antibody (11703-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

MCM5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11703-1-AP

Publications(23)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

CDC46, EC:3.6.4.12, P1 CDC46, P1-CDC46

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Tested Applications

• Positive WB detected in: A375 cells, HeLa cells, Jurkat cells, mouse thymus tissue, rat thymus tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: mouse thymus tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 18 publications below
• IHC: See 6 publications below
• IF: See 1 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

11703-1-AP targets MCM5 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2271

  Product name: Recombinant human MCM5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 388-734 aa of BC000142

  Sequence: SQLLKFVEKCSPIGVYTSGKGSSAAGLTASVMRDPSSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSVFGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLHVSALTQTQAVEGEIDLAKLKKFIAYCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDSDRRSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALRLFQVSTLDAALSGTLSGVEGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTKQKYPEHAIHKVLQLMLRRGEIQHRMQRKVLYRLK

• Full Name: minichromosome maintenance complex component 5
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 82-88 kDa
• GenBank Accession Number: BC000142
• Gene Symbol: MCM5
• Gene ID (NCBI): 4174
• RRID: AB_2235162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P33992
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Minichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,RIP,IP	Mol Ther; Long noncoding RNA lnc-POP1-1 upregulated by VN1R5 promotes cisplatin resistance in head and neck squamous cell carcinoma through interaction with MCM5.; Authors - Yingying Jiang
human	WB	Elife; MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells.; Authors - Yuichiro Saito
human	WB	J Nanobiotechnology; Graphene quantum dots rescue angiogenic retinopathy via blocking STAT3/Periostin/ERK signaling.; Authors - Na Zhao
human	WB	Front Oncol; METTL3 Promotes the Progression of Gastric Cancer via Targeting the MYC Pathway.; Authors - Dong-Dong Yang
human	WB	Oncotarget; Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.; Authors - Yinan Ma
human	WB	Ecotoxicol Environ Saf; The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis.; Authors - Zemin Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hana (Verified Customer) (07-04-2023)

In this immunoprecipitation experiment, we tested the interaction between MCMBP and MCM5 (detected by 11703-1-AP). One can observe that clear interaction between these two proteins as it was reported previously Sedlackova et al, Nature, 2020.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: human osteosarcoma U2OS

MCM5 Antibody Western Blot,Immunoprecipitation validation (1:2000 dilution) in human osteosarcoma U2OS (Cat no:11703-1-AP)