CoraLite®594-conjugated MDH1 Monoclonal antibody

• Featured Product
• KD/KO Validated

MDH1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F9A2

Cat no : CL594-66505

Synonyms

Cytosolic malate dehydrogenase, MDH s, MDH1, MDHA, MOR2

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-66505

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 MDH1 antibody (CL594-66505, Clone: 1F9A2 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66505 targets MDH1 in IF applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MDH1 fusion protein Ag8744
• Full Name: malate dehydrogenase 1, NAD (soluble)
• Calculated Molecular Weight: 334 aa, 36 kDa
• Observed Molecular Weight: 36-37 kDa
• GenBank Accession Number: BC001484
• Gene Symbol: MDH1
• Gene ID (NCBI): 4190
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme. (PMID:10693743)