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MDH1 Monoclonal antibody

MDH1 Monoclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Mouse / IgG1


Human, mouse







Cat no : 66505-1-Ig


Cytosolic malate dehydrogenase, MDH s, MDH1, MDHA, MOR2

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, NIH/3T3 cells, RAW 264.7 cells, Jurkat cells
Positive IF detected inHepG2 cells
Positive FC detected inHEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66505-1-Ig targets MDH1 in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MDH1 fusion protein Ag8744
Full Name malate dehydrogenase 1, NAD (soluble)
Calculated Molecular Weight 334 aa, 36 kDa
Observed Molecular Weight 36-37 kDa
GenBank Accession NumberBC001484
Gene Symbol MDH1
Gene ID (NCBI) 4190
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme. (PMID:10693743)


Product Specific Protocols
WB protocol for MDH1 antibody 66505-1-IgDownload protocol
IF protocol for MDH1 antibody 66505-1-IgDownload protocol
FC protocol for MDH1 antibody 66505-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols