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MDH1 Monoclonal antibody
MDH1 Monoclonal Antibody for FC, IF, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse
Applications
WB, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1F9A2
Cat no : 66505-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, NIH/3T3 cells, RAW 264.7 cells, Jurkat cells |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
66505-1-Ig targets MDH1 in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.
Tested Reactivity | Human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | MDH1 fusion protein Ag8744 |
Full Name | malate dehydrogenase 1, NAD (soluble) |
Calculated molecular weight | 334 aa, 36 kDa |
Observed molecular weight | 36-37 kDa |
GenBank accession number | BC001484 |
Gene symbol | MDH1 |
Gene ID (NCBI) | 4190 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme. (PMID:10693743)
Protocols
Product Specific Protocols | |
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WB protocol for MDH1 antibody 66505-1-Ig | Download protocol |
IF protocol for MDH1 antibody 66505-1-Ig | Download protocol |
FC protocol for MDH1 antibody 66505-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |