MDH1 Monoclonal antibody

• Featured Product
• KD/KO Validated

MDH1 Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1F9A2

Cat no : 66505-1-Ig

Synonyms

Cytosolic malate dehydrogenase, MDH s, MDH1, MDHA, MOR2

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Validation Data Gallery

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  WB analysis using 66505-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66505-1-Ig (MDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66505-1-Ig

  WB result of MDH1 antibody (66505-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDH1 transfected HEK-293 cells.

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  WB analysis of NIH/3T3 using 66505-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66505-1-Ig (MDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66505-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66505-1-Ig (MDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 66505-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66505-1-Ig (MDH1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HEK-293 using 66505-1-Ig

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human MDH1 (66505-1-Ig, Clone:1F9A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, NIH/3T3 cells, RAW 264.7 cells, Jurkat cells
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

66505-1-Ig targets MDH1 in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MDH1 fusion protein Ag8744
• Full Name: malate dehydrogenase 1, NAD (soluble)
• Calculated molecular weight: 334 aa, 36 kDa
• Observed molecular weight: 36-37 kDa
• GenBank accession number: BC001484
• Gene symbol: MDH1
• Gene ID (NCBI): 4190
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme. (PMID:10693743)