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MDH1 Monoclonal antibody

MDH1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66505-1-Ig
Clone No.1F9A2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

MDHA, KAR, EC:1.1.1.96, EC:1.1.1.37, Aromatic alpha-keto acid reductase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, NIH/3T3 cells, RAW 264.7 cells, Jurkat cells
Positive IHC detected inrat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66505-1-Ig targets MDH1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8744

Product name: Recombinant human MDH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-334 aa of BC001484

Sequence: MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKESAFEFLSSA

Predict reactive species
Full Name malate dehydrogenase 1, NAD (soluble)
Calculated Molecular Weight 334 aa, 36 kDa
Observed Molecular Weight 36-37 kDa
GenBank Accession NumberBC001484
Gene Symbol MDH1
Gene ID (NCBI) 4190
RRIDAB_2881868
Conjugate Unconjugated
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP40925
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme. (PMID:10693743)

Protocols

Product Specific Protocols
WB protocol for MDH1 antibody 66505-1-IgDownload protocol
IHC protocol for MDH1 antibody 66505-1-IgDownload protocol
IF protocol for MDH1 antibody 66505-1-IgDownload protocol
FC protocol for MDH1 antibody 66505-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Cell Mol Biol Lett

IDH1/MDH1 deacetylation promotes acute liver failure by regulating NETosis

Authors - Yukun Wang
mouseWB

J Proteome Res

Characteristics of Myocardial Structure and Central Carbon Metabolism during the Early and Compensatory Stages of Cardiac Hypertrophy

Authors - Yuan Chen
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