MFN2 Polyclonal antibody

MFN2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 28341-1-AP

Synonyms

CMT2A, CMT2A2, CPRP1, HSG, KIAA0214, MARF, MFN2, mitofusin 2, Transmembrane GTPase MFN2

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Validation Data Gallery

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  WB analysis of HepG2 using 28341-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 28341-1-AP (MFN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 28341-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28341-1-AP (MFN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 28341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28341-1-AP (MFN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 28341-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28341-1-AP (MFN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, HeLa cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28341-1-AP targets MFN2 in WB, IHC,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MFN2 fusion protein Ag28128
• Full Name: mitofusin 2
• Calculated Molecular Weight: 757 aa, 86 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC017061
• Gene Symbol: MFN2
• Gene ID (NCBI): 9927
• RRID: AB_2881116
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MFN2, also named as CPRP1 and KIAA0214, belongs to the mitofusin family. It is an Essential transmembrane GTPase, which mediates mitochondrial fusion. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression of MFN2 induces the formation of mitochondrial networks. It plays an important role in the regulation of vascular smooth muscle cell proliferation. Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 2A2 (CMT2A2). Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 6 (CMT6). Ubiquitinated forms of Mfn2 (mono- and polyubiquitinated) are present during mitophagy.