Validation Data Gallery
|Positive WB detected in||HL-60 cells, U-937 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue, mouse colon tissue, human liver tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22225-1-AP is MPO Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, rat, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MPO fusion protein Ag17564|
|Calculated molecular weight||745 aa, 84 kDa|
|Observed molecular weight||59 kDa|
|GenBank accession number||BC130476|
|Gene ID (NCBI)||4353|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing. This antibody is specific to MPO.
|Product Specific Protocols|
|WB protocol for MPO antibody 22225-1-AP||Download protocol|
|IHC protocol for MPO antibody 22225-1-AP||Download protocol|
|IF protocol for MPO antibody 22225-1-AP||Download protocol|
|IP protocol for MPO antibody 22225-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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