MPO Polyclonal antibody

MPO Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 22225-1-AP

Synonyms

MPO, myeloperoxidase



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, U-937 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, mouse colon tissue, human liver tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy MPO Ready-To-Use IHC Kit. MPO primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22225-1-AP is MPO Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MPO fusion protein Ag17564
Full Name myeloperoxidase
Calculated molecular weight 745 aa, 84 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC130476
Gene symbol MPO
Gene ID (NCBI) 4353
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing. This antibody is specific to MPO.

Protocols

Product Specific Protocols
WB protocol for MPO antibody 22225-1-APDownload protocol
IHC protocol for MPO antibody 22225-1-APDownload protocol
IF protocol for MPO antibody 22225-1-APDownload protocol
IP protocol for MPO antibody 22225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Toxicology

A new perspective of triptolide-associated hepatotoxicity: Liver hypersensitivity upon LPS stimulation.

Authors - Ziqiao Yuan
mouseIHC

Inflammation

α7-nAChR Activation Has an Opposite Effect on Healing of Covered and Uncovered Wounds.

Authors - Jiao-Yong Li
mouseIF

Toxicol Appl Pharmacol

Curcumin plays neuroprotective roles against traumatic brain injury partly via Nrf2 signaling.

Authors - Wenwen Dong
ratIF

Oncol Rep

Emodin attenuated severe acute pancreatitis via the P2X ligand‑gated ion channel 7/NOD‑like receptor protein 3 signaling pathway.

Authors - Qingkai Zhang
humanIF

Cancer Biol Med

Neutrophil extracellular traps mediate the crosstalk between glioma progression and the tumor microenvironment via the HMGB1/RAGE/IL-8 axis.

Authors - Caijun Zha
mouseWB,IHC

Acta Pharm Sin B

A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein.

Authors - Ziqiao Yuan