CD206 Polyclonal antibody

CD206 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 18704-1-AP

Synonyms

Human mannose receptor, hMR, C-type lectin domain family 13 member D-like, C-type lectin domain family 13 member D, CLEC13DL



Tested Applications

Positive WB detected inhuman placenta tissue, human kidney tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse lung tissue
Positive FC (Intra) detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18704-1-AP targets CD206 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, rabbit, mussel
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name mannose receptor, C type 1
Calculated Molecular Weight 166 kDa
Observed Molecular Weight 165-180 kDa
GenBank Accession NumberNM_002438
Gene Symbol CD206
Gene ID (NCBI) 4360
RRIDAB_10597232
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Background

CD206 (macrophage mannose receptor 1) is a lectin-type endocytic receptor expressed on selected macrophages, dendritic cells, and non-vascular endothelium and plays a role in antigen processing and presentation, phagocytosis, and intracellular signaling.

1. What is the molecular weight of CD206?

The molecular size of full-length CD206 is 170-180 kDa, depending on the exact tissue-specific glycosylation pattern (PMID: 19427834). Additionally, CD206 can be cleaved off and a soluble form (sMR) lacking the tail, with a slightly lower molecular weight, can be released to the cell medium (PMID: 9722572).

2. What is the subcellular localization of CD206?

CD206 is a type I membrane protein composed of a large extracellular multidomain, a transmembrane domain, and a short cytoplasmic tail. It is present at the plasma membrane and in endosomes, as CD206 undergoes constant recycling between the plasma membrane and endosomal compartment.

3. Is CD206 post-translationally modified?

CD206 undergoes quite extensive post-translational modifications, predominantly N-linked glycosylation that affects ligand binding recognition and affinity (PMID: 22966131).

4. Can CD206 marker be used as a marker of M2 macrophages?

The activation of macrophages with various stimuli leads to their polarization into classical (M1) or alternatively activated (M2) subtypes spectrums and both subtypes differ in their regulatory and effector functions (PMID: 24669294). Pathogens and IFN-γ promote M1 polarization, while IL-4 released during parasite infections and allergen response promotes M2 polarization. Classically, the markers of M2 macrophages include CD206, as well as arginase-1 (ARG1; https://www.ptglab.com/products/ARG1-Antibody-16001-1-AP.htm), CD163 (https://www.ptglab.com/products/CD163-Antibody-16646-1-AP.htm), and thrombospondin 1 (TSP1/ THBS1; https://www.ptglab.com/products/TSP1-Antibody-18304-1-AP.htm).

5. How can you polarize macrophages into M2 direction?

One of the most commonly used methods is stimulation by the addition of IL-4 cytokine. We recommend using our animal-free human IL-4 (https://www.ptglab.com/products/recombinant-human-il-4.htm).

Protocols

Product Specific Protocols
WB protocol for CD206 antibody 18704-1-APDownload protocol
IHC protocol for CD206 antibody 18704-1-APDownload protocol
IF protocol for CD206 antibody 18704-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nature

Inhibition of LTβR signalling activates WNT-induced regeneration in lung.

Authors - Thomas M Conlon
mouseIF

Adv Mater

Osteoimmunity-Regulating Biomimetically Hierarchical Scaffold for Augmented Bone Regeneration.

Authors - Jin Zhang
ratIF

Bioact Mater

Highly active probiotic hydrogels matrixed on bacterial EPS accelerate wound healing via maintaining stable skin microbiota and reducing inflammation

Authors - Hongtao Xu
mouse,ratIF

Bioact Mater

Reprogramming macrophages via immune cell mobilized hydrogel microspheres for osteoarthritis treatments

Authors - Pengcheng Xiao
mouseIF

ACS Nano

Glutamine Antagonist Synergizes with Electrodynamic Therapy to Induce Tumor Regression and Systemic Antitumor Immunity.

Authors - Gui Chen
humanIHC,IF

Adv Sci (Weinh)

Colorectal Cancer-Derived Small Extracellular Vesicles Promote Tumor Immune Evasion by Upregulating PD-L1 Expression in Tumor-Associated Macrophages.

Authors - Yuan Yin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Murali (Verified Customer) (07-28-2022)

This amazing antibody works well for human IHC staining.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: heart
CD206 Antibody Immunohistochemistry validation (1:50 dilution) in heart (Cat no:18704-1-AP)
FH

Uthra (Verified Customer) (10-14-2021)

The antibody works fine for IF.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:150
  • Cell Tissue Type: heart
CD206 Antibody Immunofluorescence validation (1:150 dilution) in heart (Cat no:18704-1-AP)
FH

Nethaji (Verified Customer) (05-08-2019)

The antibody works fine for western and ICC.

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: cell culture