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- KD/KO Validated
MUC16,CA125 Polyclonal antibody
MUC16,CA125 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 20077-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
Product Information
20077-1-AP targets MUC16,CA125 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | mucin 16, cell surface associated |
| Calculated Molecular Weight | 1519 kDa |
| Observed Molecular Weight | 90-100 kDa |
| GenBank Accession Number | NM_024690 |
| Gene Symbol | MUC16 |
| Gene ID (NCBI) | 94025 |
| RRID | AB_10695048 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MUC16, also named as CA125, is thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. It is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller "subunits" being described by some investigators. The antibody recognizes the C-term of MUC16.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MUC16,CA125 antibody 20077-1-AP | Download protocol |
| IHC protocol for MUC16,CA125 antibody 20077-1-AP | Download protocol |
| IP protocol for MUC16,CA125 antibody 20077-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Sci Carcinoembryonic antigen as a specific glycoprotein ligand of rBC2LCN lectin on pancreatic ductal adenocarcinoma cells. | ||
Toxicology Necroptosis contributes to airborne particulate matter-induced ocular surface injury. | ||
Biochem Biophys Res Commun MUC16 mutation predicts a favorable clinical outcome and correlates decreased Warburg effect in gastric cancer. | ||
J Exp Clin Cancer Res Dysregulation and prometastatic function of glycosyltransferase C1GALT1 modulated by cHP1BP3/ miR-1-3p axis in bladder cancer.
| ||
Cancers (Basel) Protein Panel of Serum-Derived Small Extracellular Vesicles for the Screening and Diagnosis of Epithelial Ovarian Cancer |
