MUC16,CA125 Polyclonal antibody

MUC16,CA125 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20077-1-AP

Synonyms

CA 125, CA125, MUC 16, MUC16, Mucin 16



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20077-1-AP targets MUC16,CA125 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name mucin 16, cell surface associated
Calculated molecular weight 1519 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberNM_024690
Gene symbol MUC16
Gene ID (NCBI) 94025
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MUC16, also named as CA125, is thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. It is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller "subunits" being described by some investigators. The antibody recognizes the C-term of MUC16.

Protocols

Product Specific Protocols
WB protocol for MUC16,CA125 antibody 20077-1-APDownload protocol
IHC protocol for MUC16,CA125 antibody 20077-1-APDownload protocol
IP protocol for MUC16,CA125 antibody 20077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

MUC16 mutation predicts a favorable clinical outcome and correlates decreased Warburg effect in gastric cancer.

Authors - Huijin Zhao
humanWB

Int J Oncol

Lewis antigen‑negative pancreatic cancer: An aggressive subgroup.

Authors - Chen Liu
humanIHC

Cancer Sci

Carcinoembryonic antigen as a specific glycoprotein ligand of rBC2LCN lectin on pancreatic ductal adenocarcinoma cells.

Authors - Tomoaki Furuta