MXI1 Recombinant antibody

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  IF Staining of SH-SY5Y using 84733-6-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using MXI1 antibody (84733-6-RR, Clone: 242089D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 84733-6-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MXI1 Recombinant antibody (84733-6-RR, Clone:242089D5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 84733-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84733-6-RR against Human MXI1 were performed. The affinity constant is below 1 pM.

MXI1 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Cat No. 84733-6-RR

Clone No.242089D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

MAX interactor 1, Max interacting protein 1, MAD2, Class C basic helix-loop-helix protein 11, bHLHc11

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Tested Applications

• Positive IF/ICC detected in: SH-SY5Y cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84733-6-RR targets MXI1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MXI1 fusion protein Ag3024
• Full Name: MAX interactor 1
• Calculated Molecular Weight: 228 aa, 26 kDa
• GenBank Accession Number: BC012907
• Gene Symbol: MXI1
• Gene ID (NCBI): 4601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P50539
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MXI1 is a transcription factor containing a basic helix-loop-helix leucine zipper (bHLHzip) and belongs to the Myc-Max-Mad transcriptional network. It is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors.