MYO18B Polyclonal antibody

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  IHC staining of mouse heart using 25691-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25691-1-AP (MYO18B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 25691-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25691-1-AP (MYO18B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 25691-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MYO18B antibody (25691-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

MYO18B Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 25691-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

MYO18B, myosin XVIIIB

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Tested Applications

• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25691-1-AP targets MYO18B in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22454

  Product name: Recombinant human MYO18B protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 617-795 aa of BC150626

  Sequence: SVPSAGKVPKGRRDGLPAHIGSMAQRAYWALLNQRRDQSIVALGRSGAGKTTCCEQVLEHLVGMAGSVDGRVSVEKIRATFTVLRAFGSVSMAHSRSATRFSMVMSLDFNATGRITAAQLQTMLLEKSRVARQPEGESNFLVFSQMLAGLDLDLRTELNLHQMADSSSFGMGVWSKPED

• Full Name: myosin XVIIIB
• Calculated Molecular Weight: 2566 aa, 285 kDa
• Observed Molecular Weight: 280-290 kDa
• GenBank Accession Number: BC150626
• Gene Symbol: MYO18B
• Gene ID (NCBI): 84700
• RRID: AB_3085815
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IUG5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.