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MYO18B Polyclonal antibody

MYO18B Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25691-1-AP

Synonyms

MYO18B, myosin XVIIIB

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse heart tissue using MYO18B Polyclonal antibody (25691-1-AP)

    IHC staining of mouse heart using 25691-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25691-1-AP (MYO18B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MYO18B Polyclonal antibody (25691-1-AP)

    IHC staining of mouse heart using 25691-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25691-1-AP (MYO18B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MYO18B Polyclonal antibody (25691-1-AP)

    IF Staining of U2OS using 25691-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MYO18B antibody (25691-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25691-1-AP targets MYO18B in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYO18B fusion protein Ag22454
Full Name myosin XVIIIB
Calculated Molecular Weight 2566 aa, 285 kDa
Observed Molecular Weight280-290 kDa
GenBank Accession NumberBC150626
Gene Symbol MYO18B
Gene ID (NCBI) 84700
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for MYO18B antibody 25691-1-APDownload protocol
IF protocol for MYO18B antibody 25691-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols