CoraLite®594-conjugated Myosin Light Chain 2 Monoclonal antibody

Myosin Light Chain 2 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5A1F1

Cat no : CL594-60229

Synonyms

MLC 2v, MYL2, MLC-2s/v, MLC-2, MLC2

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Validation Data Gallery

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  IF Staining of mouse heart using CL594-60229

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite®594 Myosin Light Chain 2 antibody (CL594-60229, Clone: 5A1F1 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using CL594-60229

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CL594-60229 (Myosin Light Chain 2 antibody) at dilution of 1:50. .

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Tested Applications

• Positive IF-P detected in: mouse heart tissue
• Positive IF/ICC detected in: C2C12 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60229 targets Myosin Light Chain 2 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Myosin Light Chain 2 fusion protein Ag1356
• Full Name: myosin, light chain 2, regulatory, cardiac, slow
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC015821
• Gene Symbol: Myosin Light Chain 2
• Gene ID (NCBI): 4633
• RRID: AB_2883447
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.