Myosin Light Chain 2/MLC-2V Monoclonal antibody
Myosin Light Chain 2/MLC-2V Monoclonal Antibody for IF, IHC, WB,ELISA
Cat no : 60229-1-Ig
|Positive WB detected in||human heart tissue, rat heart tissue, pig heart tissue|
|Positive IHC detected in||human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||C2C12 cell|
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 60229-1-Ig is Myosin Light Chain 2/MLC-2V Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Myosin Light Chain 2/MLC-2V fusion protein Ag1356|
|Full Name||myosin, light chain 2, regulatory, cardiac, slow|
|Calculated molecular weight||19 kDa|
|Observed molecular weight||18 kDa|
|GenBank accession number||BC015821|
|Gene ID (NCBI)||4633|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.
|Product Specific Protocols|
|WB protocol for Myosin Light Chain 2/MLC-2V antibody 60229-1-Ig||Download protocol|
|IHC protocol for Myosin Light Chain 2/MLC-2V antibody 60229-1-Ig||Download protocol|
|IF protocol for Myosin Light Chain 2/MLC-2V antibody 60229-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Stem Cell Rev Rep
MYOCD is Required for Cardiomyocyte-like Cells Induction from Human Urine Cells and Fibroblasts Through Remodeling Chromatin.
Nitric Oxide-cGMP-PKG Pathway Acts on Orai1 to Inhibit the Hypertrophy of Human Embryonic Stem Cell-Derived Cardiomyocytes.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Irem (Verified Customer) (04-19-2022)
Unfortunately, this antibody unspecifically binds to many proteins in Jurkat cells.