Myosin Light Chain 2/MLC-2V Monoclonal antibody
Myosin Light Chain 2/MLC-2V Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 60229-1-Ig
Validation Data Gallery
|Positive WB detected in||human heart tissue, pig heart tissue, rat heart tissue, rabbit heart tissue|
|Positive IHC detected in||mouse heart tissue, mouse skeletal muscle tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||C2C12 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
60229-1-Ig targets Myosin Light Chain 2/MLC-2V in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
|Tested Reactivity||human, mouse, rat, pig, rabbit|
|Cited Reactivity||human, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Myosin Light Chain 2/MLC-2V fusion protein Ag1356|
|Full Name||myosin, light chain 2, regulatory, cardiac, slow|
|Calculated molecular weight||19 kDa|
|Observed molecular weight||18 kDa|
|GenBank accession number||BC015821|
|Gene ID (NCBI)||4633|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.
|Product Specific Protocols|
|WB protocol for Myosin Light Chain 2/MLC-2V antibody 60229-1-Ig||Download protocol|
|IHC protocol for Myosin Light Chain 2/MLC-2V antibody 60229-1-Ig||Download protocol|
|IF protocol for Myosin Light Chain 2/MLC-2V antibody 60229-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Nitric Oxide-cGMP-PKG Pathway Acts on Orai1 to Inhibit the Hypertrophy of Human Embryonic Stem Cell-Derived Cardiomyocytes.
Int J Stem Cells
Synergistic Effect of Hydrogen and 5-Aza on Myogenic Differentiation through the p38 MAPK Signaling Pathway in Adipose-Derived Mesenchymal Stem Cells
Stem Cell Rev Rep
MYOCD is Required for Cardiomyocyte-like Cells Induction from Human Urine Cells and Fibroblasts Through Remodeling Chromatin.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Irem (Verified Customer) (04-19-2022)
Unfortunately, this antibody unspecifically binds to many proteins in Jurkat cells.