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NF-L Monoclonal antibody

NF-L Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

5C12G4

Cat no : 60189-1-Ig

Synonyms

68 kDa neurofilament protein, NEFL, Neurofilament L, NF, NF68, NFL, NF-L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NF-L Monoclonal antibody (60189-1-Ig)

    WB analysis using 60189-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60189-1-Ig (NF-L antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using NF-L Monoclonal antibody (60189-1-Ig)

    IHC staining of rat brain using 60189-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NF-L Monoclonal antibody (60189-1-Ig)

    IHC staining of mouse brain using 60189-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using NF-L Monoclonal antibody (60189-1-Ig)

    IHC staining of human brain using 60189-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NF-L Monoclonal antibody (60189-1-Ig)

    IF Staining of mouse brain using 60189-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 60189-1-Ig (NF-L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using NF-L Monoclonal antibody (60189-1-Ig)

    IF Staining of rat brain using 60189-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NF-L antibody (60189-1-Ig, Clone: 5C12G4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of PC-12 cells using NF-L Monoclonal antibody (60189-1-Ig)

    FC experiment of PC-12 using 60189-1-Ig

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-L (60189-1-Ig, Clone:5C12G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inPC-12 cells, human brain tissue, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inrat brain tissue, human brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, rat brain tissue
Positive FC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60189-1-Ig targets NF-L in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NF-L fusion protein Ag15178
Full Name neurofilament, light polypeptide
Calculated molecular weight 543 aa, 62 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC039237
Gene symbol NEFL
Gene ID (NCBI) 4747
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL.

Protocols

Product Specific Protocols
WB protocol for NF-L antibody 60189-1-IgDownload protocol
IHC protocol for NF-L antibody 60189-1-IgDownload protocol
IF protocol for NF-L antibody 60189-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols