Neuropeptide Y Polyclonal antibody

Neuropeptide Y Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12833-1-AP

Synonyms

CPON, Neuropeptide tyrosine, neuropeptide Y, NPY, PYY4



Tested Applications

Positive IHC detected inhuman brain tissue, mouse brain tissue, human pancreas cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, mouse eye tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12833-1-AP targets Neuropeptide Y in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Neuropeptide Y fusion protein Ag3568
Full Name neuropeptide Y
Calculated Molecular Weight 97 aa, 11 kDa
GenBank Accession NumberBC029497
Gene Symbol NPY
Gene ID (NCBI) 4852
RRIDAB_10791890
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuropeptide Y (NPY) is one of the most abundant peptides in the mammalian central nervous system (CNS). NPY has been linked to several physiological and pathological functions, such as feeding behaviour, memory processing, pain, anxiety, cell proliferation and many other processes in the central and peripheral nervous systems. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. In addition, NPY was suggested as a potential neuroprotective agent in Alzheimer's disease by counteracting the toxic effect of β-amyloid in an in vitro model.

Protocols

Product Specific Protocols
IHC protocol for Neuropeptide Y antibody 12833-1-APDownload protocol
IF protocol for Neuropeptide Y antibody 12833-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Free Radic Biol Med

Inhibition of neuronal nitric oxide synthase protects against hippocampal neuronal injuries by increasing neuropeptide Y expression in temporal lobe epilepsy mice.

Authors - Yuanyuan Yao
humanIHC

J Orthop Translat

Suprascapular nerve injury affects rotator cuff healing: A paired controlled study in a rat model.

Authors - Yucheng Sun
ratIF

Front Behav Neurosci

Heterogeneous GAD65 Expression in Subtypes of GABAergic Neurons Across Layers of the Cerebral Cortex and Hippocampus.

Authors - Yuki Kajita

Acta Neurobiol Exp (Wars)

Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor rat.

Authors - Xiaoxue Xu
humanIHC

Acta Histochem

Loss of nerve fibers in the oviduct isthmus in women with hydrosalpinx.

Authors - Zhu Libo L
humanIHC

Acta Histochem

Decreased nerve fibers in the oviduct isthmus of women with endometriosis.

Authors - Libo Zhu