Neuropilin 2 Recombinant monoclonal antibody

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  WB analysis of U-87 MG using 86126-3-RR

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 86126-3-RR (NRP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of U-87 MG using 86126-3-RR

  Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using Neuropilin 2 antibody (86126-3-RR, Clone: 250688C6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Neuropilin 2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86126-3-RR

Clone No.250688C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

NRP2, Neuropilin-2

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Tested Applications

• Positive WB detected in: U-87 MG cells
• Positive IF/ICC detected in: U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86126-3-RR targets Neuropilin 2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3125

  Product name: Recombinant Human Neuropilin 2 protein (rFc Tag)(HPLC verified)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 21-864 aa of NM_201266.1

  Sequence: RGQPDPPCGGRLNSKDAGYITSPGYPQDYPSHQNCEWIVYAPEPNQKIVLNFNPHFEIEKHDCKYDFIEIRDGDSESADLLGKHCGNIAPPTIISSGSMLYIKFTSDYARQGAGFSLRYEIFKTGSEDCSKNFTSPNGTIESPGFPEKYPHNLDCTFTILAKPKMEIILQFLIFDLEHDPLQVGEGDCKYDWLDIWDGIPHVGPLIGKYCGTKTPSELRSSTGILSLTFHTDMAVAKDGFSARYYLVHQEPLENFQCNVPLGMESGRIANEQISASSTYSDGRWTPQQSRLHGDDNGWTPNLDSNKEYLQVDLRFLTMLTAIATQGAISRETQNGYYVKSYKLEVSTNGEDWMVYRHGKNHKVFQANNDATEVVLNKLHAPLLTRFVRIRPQTWHSGIALRLELFGCRVTDAPCSNMLGMLSGLIADSQISASSTQEYLWSPSAARLVSSRSGWFPRIPQAQPGEEWLQVDLGTPKTVKGVIIQGARGGDSITAVEARAFVRKFKVSYSLNGKDWEYIQDPRTQQPKLFEGNMHYDTPDIRRFDPIPAQYVRVYPERWSPAGIGMRLEVLGCDWTDSKPTVETLGPTVKSEETTTPYPTEEEATECGENCSFEDDKDLQLPSGFNCNFDFLEEPCGWMYDHAKWLRTTWASSSSPNDRTFPDDRNFLRLQSDSQREGQYARLISPPVHLPRSPVCMEFQYQATGGRGVALQVVREASQESKLLWVIREDQGGEWKHGRIILPSYDMEYQIVFEGVIGKGRSGEIAIDDIRISTDVPLENCMEPISAFAGENFKVDIPEIHEREGYEDEIDDEYEVDWSNSSSATSGSGAPSTDKEKSWLYTLDP

• Full Name: neuropilin 2
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: NM_201266.1
• Gene Symbol: Neuropilin 2
• Gene ID (NCBI): 8828
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60462-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuropilin 2 (NRP2) is a single-chain transmembrane glycoprotein that acts as a co-receptor of VEGF. NRP2 is mainly expressed in lymphatic and venous endothelial cells, and it is associated with the occurrence and development of several types of cancer. NRP2 is known to play a role in cardiovascular development, axon guidance, tumorigenesis, inflammation and cardiovascular disease.