ORAI2 Polyclonal antibody, PBS Only

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  WB analysis of MCF-7 using 20592-1-AP (same clone as 20592-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20592-1-AP (ORAI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20592-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 20592-1-AP (same clone as 20592-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20592-1-AP (ORAI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20592-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 20592-1-AP (same clone as 20592-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20592-1-AP (ORAI2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 20592-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 20592-1-AP (same clone as 20592-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20592-1-AP (ORAI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20592-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 20592-1-AP (same clone as 20592-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ORAI2 antibody (20592-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20592-1-PBS in a different storage buffer formulation.

ORAI2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 20592-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C7orf19, CAP-binding protein complex-interacting protein 2, CBCIP2, PP1729, Protein orai 2

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Product Information

20592-1-PBS targets ORAI2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14540

  Product name: Recombinant human ORAI2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 176-254 aa of BC069270

  Sequence: VDARRQPGPPPGPGSHTGWQAALVSTIIMVPVGLIFVVFTIHFYRSLVRHKTERHNREIEELHKLKVQLDGHERSLQVL

• Full Name: ORAI calcium release-activated calcium modulator 2
• Calculated Molecular Weight: 254 aa, 29 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC069270
• Gene Symbol: ORAI2
• Gene ID (NCBI): 80228
• RRID: AB_2878705
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q96SN7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ORAI2, also named as C7orf19, CBCIP2, TMEM142B and PP1729, is a Ca2+ release-activated Ca2+-like (CRAC-like) channel subunit. The MW of ORAI2 is about 36 kDa for glycosylation (PMID: 19675303).