ORAI2 Polyclonal antibody

ORAI2 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 20592-1-AP

Synonyms

C7orf19, CBCIP2, ORAI2, Protein orai 2, TMEM142B, Transmembrane protein 142B



Tested Applications

Positive WB detected inMCF-7 cells, mouse heart tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20592-1-AP is ORAI2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ORAI2 fusion protein Ag14540
Full Name ORAI calcium release-activated calcium modulator 2
Calculated molecular weight 254 aa, 29 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC069270
Gene symbol ORAI2
Gene ID (NCBI) 80228
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ORAI2, also named as C7orf19, CBCIP2, TMEM142B and PP1729, is a Ca2+ release-activated Ca2+-like (CRAC-like) channel subunit. The MW of ORAI2 is about 36 kDa for glycosylation (PMID: 19675303).

Protocols

Product Specific Protocols
WB protocol for ORAI2 antibody 20592-1-APDownload protocol
IHC protocol for ORAI2 antibody 20592-1-APDownload protocol
IF protocol for ORAI2 antibody 20592-1-APDownload protocol
FC protocol for ORAI2 antibody 20592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Exp Ther Med

Resveratrol sensitizes A549 cells to irradiation damage via suppression of store-operated calcium entry with Orai1 and STIM1 downregulation.

Authors - Wu Lele
mouseIP

BMJ Open Diabetes Res Care

Orai-vascular endothelial-cadherin signaling complex regulates high-glucose exposure-induced increased permeability of mouse aortic endothelial cells.

Authors - Yuan Wei
mouseWB

J Am Heart Assoc

Cardiac-Specific Deletion of Orai3 Leads to Severe Dilated Cardiomyopathy and Heart Failure in Mice.

Authors - Jesse Gammons