Tested Applications
| Positive WB detected in | MCF-7 cells, mouse heart tissue |
| Positive IHC detected in | rat brain tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20592-1-AP targets ORAI2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14540 Product name: Recombinant human ORAI2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 176-254 aa of BC069270 Sequence: VDARRQPGPPPGPGSHTGWQAALVSTIIMVPVGLIFVVFTIHFYRSLVRHKTERHNREIEELHKLKVQLDGHERSLQVL Predict reactive species |
| Full Name | ORAI calcium release-activated calcium modulator 2 |
| Calculated Molecular Weight | 254 aa, 29 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC069270 |
| Gene Symbol | ORAI2 |
| Gene ID (NCBI) | 80228 |
| RRID | AB_2878705 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q96SN7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ORAI2, also named as C7orf19, CBCIP2, TMEM142B and PP1729, is a Ca2+ release-activated Ca2+-like (CRAC-like) channel subunit. The MW of ORAI2 is about 36 kDa for glycosylation (PMID: 19675303).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ORAI2 antibody 20592-1-AP | Download protocol |
| IHC protocol for ORAI2 antibody 20592-1-AP | Download protocol |
| WB protocol for ORAI2 antibody 20592-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ORAI2 Polyclonal antibody (Cat# 20592-1-AP) has 10 citations across journals including Cell Communications & Signaling, Annals of Neurology, Journal of the American Heart Association, Frontiers in Molecular Biosciences, BMJ Open Diabetes Research & Care, Allergy Asthma Immunology Research, Phytochemistry, Experimental & Therapeutic Medicine, Ecotoxicology and Environmental Safety, and Journal of Orthopaedic Translation. Research fields span calcium signaling, neurodegeneration, cardiovascular disease, asthma, vascular biology, and cancer.
| Species | Application | Title |
|---|---|---|
Cell Commun Signal Integrin activation by two independently regulated calcium-mediated pathways is required for neutrophil recruitment. | ||
J Orthop Translat Tibial cortex transverse transport regulates Orai1/STIM1-mediated NO release and improve the migration and proliferation of vessels via increasing osteopontin expression | ||
Ann Neurol Resilience to Endoplasmic Reticulum Stress Mitigates Membrane Hyperexcitability Underlying Late Disease Onset in a Murine Model of SCA6 | ||
Ecotoxicol Environ Saf High cadmium exposure impairs adult hippocampal neurogenesis via disruption of store-operated calcium entry | ||
J Am Heart Assoc Cardiac-Specific Deletion of Orai3 Leads to Severe Dilated Cardiomyopathy and Heart Failure in Mice. | ||
Front Mol Biosci X-Ray Causes mRNA Transcripts Change to Enhance Orai2-Mediated Ca2+ Influx in Rat Brain Microvascular Endothelial Cells. |
Reviews
What customers say
The product has one review, rated 5 stars. The reviewer used it for Western Blot on heart tissue and reported that it "works great," indicating strong performance and satisfaction with the antibody's reliability in this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marcel (Verified Customer) (03-20-2023) | Works great.
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