ORAI2 Polyclonal antibody

ORAI2 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20592-1-AP

Synonyms

C7orf19, CBCIP2, ORAI2, Protein orai 2, TMEM142B, Transmembrane protein 142B



Tested Applications

Positive WB detected inMCF-7 cells, mouse heart tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20592-1-AP is ORAI2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ORAI2 fusion protein Ag14540
Full Name ORAI calcium release-activated calcium modulator 2
Calculated molecular weight 254 aa, 29 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC069270
Gene symbol ORAI2
Gene ID (NCBI) 80228
RRIDAB_2878705
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ORAI2, also named as C7orf19, CBCIP2, TMEM142B and PP1729, is a Ca2+ release-activated Ca2+-like (CRAC-like) channel subunit. The MW of ORAI2 is about 36 kDa for glycosylation (PMID: 19675303).

Protocols

Product Specific Protocols
WB protocol for ORAI2 antibody 20592-1-APDownload protocol
IHC protocol for ORAI2 antibody 20592-1-APDownload protocol
IF protocol for ORAI2 antibody 20592-1-APDownload protocol
FC protocol for ORAI2 antibody 20592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Am Heart Assoc

Cardiac-Specific Deletion of Orai3 Leads to Severe Dilated Cardiomyopathy and Heart Failure in Mice.

Authors - Jesse Gammons
ratWB

Front Mol Biosci

X-Ray Causes mRNA Transcripts Change to Enhance Orai2-Mediated Ca2+ Influx in Rat Brain Microvascular Endothelial Cells.

Authors - Fangfang Xu
mouseIP

BMJ Open Diabetes Res Care

Orai-vascular endothelial-cadherin signaling complex regulates high-glucose exposure-induced increased permeability of mouse aortic endothelial cells.

Authors - Yuan Wei
humanWB

Exp Ther Med

Resveratrol sensitizes A549 cells to irradiation damage via suppression of store-operated calcium entry with Orai1 and STIM1 downregulation.

Authors - Wu Lele
mouseWB

Phytochemistry

Bisbenzylisoquinoline alkaloids from Plumula Nelumbinis inhibit vascular smooth muscle cells migration and proliferation by regulating the ORAI2/Akt pathway

Authors - Xiao Feng
ratWB

Allergy Asthma Immunol Res

Exercise Reduces Airway Smooth Muscle Contraction in Asthmatic Rats via Inhibition of IL-4 Secretion and Store-Operated Ca2+ Entry Pathway

Authors - Jun-Hao Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Marcel (Verified Customer) (03-20-2023)

Works great.

  • Applications: Western Blot
  • Cell Tissue Type: Heart