ORAI2 Polyclonal antibody

ORAI2 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 20592-1-AP

Synonyms

C7orf19, CBCIP2, ORAI2, Protein orai 2, TMEM142B, Transmembrane protein 142B

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Validation Data Gallery

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  WB analysis of MCF-7 using 20592-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20592-1-AP (ORAI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 20592-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20592-1-AP (ORAI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 20592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20592-1-AP (ORAI2 Antibody) at dilution of 1:50.

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  IF Staining of HEK-293 using 20592-1-AP

  Immunofluorescent analysis of HEK-293 cells using 20592-1-AP (ORAI2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of HEK-293 using 20592-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug ORAI2 antibody (20592-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse heart tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells
• Positive FC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 5 publications below
• IP: See 1 publications below

Product Information

The immunogen of 20592-1-AP is ORAI2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ORAI2 fusion protein Ag14540
• Full Name: ORAI calcium release-activated calcium modulator 2
• Calculated molecular weight: 254 aa, 29 kDa
• Observed molecular weight: 36 kDa
• GenBank accession number: BC069270
• Gene symbol: ORAI2
• Gene ID (NCBI): 80228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ORAI2, also named as C7orf19, CBCIP2, TMEM142B and PP1729, is a Ca2+ release-activated Ca2+-like (CRAC-like) channel subunit. The MW of ORAI2 is about 36 kDa for glycosylation (PMID: 19675303).

Publications

Species	Application	Title
mouse	WB	J Am Heart Assoc; Cardiac-Specific Deletion of Orai3 Leads to Severe Dilated Cardiomyopathy and Heart Failure in Mice.; Authors - Jesse Gammons
rat	WB	Front Mol Biosci; X-Ray Causes mRNA Transcripts Change to Enhance Orai2-Mediated Ca2+ Influx in Rat Brain Microvascular Endothelial Cells.; Authors - Fangfang Xu
mouse	IP	BMJ Open Diabetes Res Care; Orai-vascular endothelial-cadherin signaling complex regulates high-glucose exposure-induced increased permeability of mouse aortic endothelial cells.; Authors - Yuan Wei
human	WB	Exp Ther Med; Resveratrol sensitizes A549 cells to irradiation damage via suppression of store-operated calcium entry with Orai1 and STIM1 downregulation.; Authors - Wu Lele
mouse	WB	Phytochemistry; Bisbenzylisoquinoline alkaloids from Plumula Nelumbinis inhibit vascular smooth muscle cells migration and proliferation by regulating the ORAI2/Akt pathway; Authors - Xiao Feng
rat	WB	Allergy Asthma Immunol Res; Exercise Reduces Airway Smooth Muscle Contraction in Asthmatic Rats via Inhibition of IL-4 Secretion and Store-Operated Ca2+ Entry Pathway; Authors - Jun-Hao Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marcel (Verified Customer) (03-20-2023)

Works great.

• Applications: Western Blot
• Cell Tissue Type: Heart