|Positive WB detected in
|MCF-7 cells, mouse heart tissue
|Positive IHC detected in
|human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:500-1:1000
|IHC : 1:20-1:200
|IF : 1:10-1:100
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
The immunogen of 20592-1-AP is ORAI2 Fusion Protein expressed in E. coli.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|ORAI2 fusion protein Ag14540
|ORAI calcium release-activated calcium modulator 2
|Calculated molecular weight
|254 aa, 29 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen Affinity purified
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
ORAI2, also named as C7orf19, CBCIP2, TMEM142B and PP1729, is a Ca2+ release-activated Ca2+-like (CRAC-like) channel subunit. The MW of ORAI2 is about 36 kDa for glycosylation (PMID: 19675303).
|Product Specific Protocols
|WB protocol for ORAI2 antibody 20592-1-AP
|IHC protocol for ORAI2 antibody 20592-1-AP
|IF protocol for ORAI2 antibody 20592-1-AP
|FC protocol for ORAI2 antibody 20592-1-AP
|Click here to view our Standard Protocols
J Am Heart Assoc
Cardiac-Specific Deletion of Orai3 Leads to Severe Dilated Cardiomyopathy and Heart Failure in Mice.
Front Mol Biosci
X-Ray Causes mRNA Transcripts Change to Enhance Orai2-Mediated Ca2+ Influx in Rat Brain Microvascular Endothelial Cells.
BMJ Open Diabetes Res Care
Orai-vascular endothelial-cadherin signaling complex regulates high-glucose exposure-induced increased permeability of mouse aortic endothelial cells.
Exp Ther Med
Resveratrol sensitizes A549 cells to irradiation damage via suppression of store-operated calcium entry with Orai1 and STIM1 downregulation.
Bisbenzylisoquinoline alkaloids from Plumula Nelumbinis inhibit vascular smooth muscle cells migration and proliferation by regulating the ORAI2/Akt pathway
Allergy Asthma Immunol Res
Exercise Reduces Airway Smooth Muscle Contraction in Asthmatic Rats via Inhibition of IL-4 Secretion and Store-Operated Ca2+ Entry Pathway
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Marcel (Verified Customer) (03-20-2023)