ORAI2 Polyclonal antibody

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  WB analysis of MCF-7 using 20592-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20592-1-AP (ORAI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 20592-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20592-1-AP (ORAI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 20592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20592-1-AP (ORAI2 Antibody) at dilution of 1:50.

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  IF Staining of HEK-293 using 20592-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ORAI2 antibody (20592-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ORAI2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 20592-1-AP

Publications(8)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

C7orf19, CAP-binding protein complex-interacting protein 2, CBCIP2, PP1729, Protein orai 2

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse heart tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

20592-1-AP targets ORAI2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14540

  Product name: Recombinant human ORAI2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 176-254 aa of BC069270

  Sequence: VDARRQPGPPPGPGSHTGWQAALVSTIIMVPVGLIFVVFTIHFYRSLVRHKTERHNREIEELHKLKVQLDGHERSLQVL

• Full Name: ORAI calcium release-activated calcium modulator 2
• Calculated Molecular Weight: 254 aa, 29 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC069270
• Gene Symbol: ORAI2
• Gene ID (NCBI): 80228
• RRID: AB_2878705
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q96SN7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ORAI2, also named as C7orf19, CBCIP2, TMEM142B and PP1729, is a Ca2+ release-activated Ca2+-like (CRAC-like) channel subunit. The MW of ORAI2 is about 36 kDa for glycosylation (PMID: 19675303).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	J Am Heart Assoc; Cardiac-Specific Deletion of Orai3 Leads to Severe Dilated Cardiomyopathy and Heart Failure in Mice.; Authors - Jesse Gammons
rat	WB	Front Mol Biosci; X-Ray Causes mRNA Transcripts Change to Enhance Orai2-Mediated Ca2+ Influx in Rat Brain Microvascular Endothelial Cells.; Authors - Fangfang Xu
mouse	IP	BMJ Open Diabetes Res Care; Orai-vascular endothelial-cadherin signaling complex regulates high-glucose exposure-induced increased permeability of mouse aortic endothelial cells.; Authors - Yuan Wei
human	WB	Exp Ther Med; Resveratrol sensitizes A549 cells to irradiation damage via suppression of store-operated calcium entry with Orai1 and STIM1 downregulation.; Authors - Wu Lele
mouse	WB	Phytochemistry; Bisbenzylisoquinoline alkaloids from Plumula Nelumbinis inhibit vascular smooth muscle cells migration and proliferation by regulating the ORAI2/Akt pathway; Authors - Xiao Feng
rat	WB	Allergy Asthma Immunol Res; Exercise Reduces Airway Smooth Muscle Contraction in Asthmatic Rats via Inhibition of IL-4 Secretion and Store-Operated Ca2+ Entry Pathway; Authors - Jun-Hao Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marcel (Verified Customer) (03-20-2023)

Works great.

• Applications: Western Blot
• Cell Tissue Type: Heart