PAM Recombinant monoclonal antibody

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• PAM Recombinant monoclonal antibody (87921-1-RR)

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  WB analysis using 87921-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87921-1-RR (PAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 87921-1-RR

  SK-N-SH cells were subjected to SDS PAGE followed by western blot with 87921-1-RR (PAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of U2OS using 87921-1-RR

  IP result of anti-PAM (IP:87921-1-RR, 4ug; Detection:87921-1-RR 1:1000) with U2OS cells lysate 800 ug.

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  IF Staining of U2OS using 87921-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PAM antibody (87921-1-RR, Clone: 260074A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity of 87921-1-RR

  Biolayer interferometry (BLI) kinetic assays of 87921-1-RR against Human PAM were performed. The affinity constant is below 1 pM.

PAM Uni-rAb^® Recombinant Antibody for WB, IF/ICC, IP, ELISA

Cat No. 87921-1-RR

Clone No.260074A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

PAL, PAM-1, Peptidyl-alpha-hydroxyglycine alpha-amidating lyase, Peptidyl-glycine alpha-amidating monooxygenase, Peptidylglycine alpha-hydroxylating monooxygenase

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Tested Applications

• Positive WB detected in: HeLa cells, U-251 cells, RD cells, U2OS cells, SK-N-SH cells
• Positive IP detected in: U2OS cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87921-1-RR targets PAM in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5194

  Product name: Recombinant human PAM protein

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 21-494 aa of NM_001177306.2

  Sequence: FRSPLSVFKRFKETTRPFSNECLGTTRPVVPIDSSDFALDIRMPGVTPKQSDTYFCMSMRIPVDEEAFVIDFKPRASMDTVHHMLLFGCNMPSSTGSYWFCDEGTCTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNNKDCSGVSLHLTRLPQPLIAGMYLMMSVDTVIPAGEKVVNSDISCHYKNYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVGHPVDVSFGDLLAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVAPDMFRTIPPEANIPIPVKSDMVMMHEHHKETEYKDKIPLLQQPKREEEEVLDQGDFYSLLSKLLGEREDVVHVHKYNPTEKAESESDLVAEIANVVQKKDLGRSDAREGAEHERGNAILVRDRIHKFHRLVSTLRPPESRVFSLQQPPPGEGTWEPEHTG

• Full Name: peptidylglycine alpha-amidating monooxygenase
• Calculated Molecular Weight: 108 kDa
• Observed Molecular Weight: 75 kDa and 105-120 kDa
• GenBank Accession Number: NM_001177306.2
• Gene Symbol: PAM
• Gene ID (NCBI): 5066
• RRID: AB_3745828
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19021-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peptidyl-glycine alpha-amidating monooxygenase (PAM) is a bifunctional type I membrane protein essential for neuropeptide maturation. It catalyzes the C-terminal amidation of peptides via two sequential enzymatic domains: peptidylglycine α-hydroxylating monooxygenase (PHM, ~44-46 kDa) and peptidyl-α-hydroxyglycine α-amidating lyase (PAL, ~50 kDa), which together convert glycine-extended precursors into bioactive amidated peptides. PAM undergoes alternative splicing, generating integral membrane isoforms such as PAM-1 (~120 kDa) and PAM-2 (~105 kDa). Endoproteolytic cleavage produces soluble monofunctional PHM and PAL, as well as a bifunctional ~75 kDa fragment. The cleaved cytosolic domain (sfCD, ~16 kDa) can translocate to the nucleus, influencing gene expression. PAM also supports angiogenesis and tumor progression, notably in glioblastoma, highlighting its therapeutic potential (PMID: 8436961; PMID: 32054826).