CoraLite® Plus 488-conjugated PAX8 Recombinant antibody

PAX8 Recombinant Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6B14

Cat no : CL488-80756

Synonyms

paired box 8, Paired box protein Pax 8, PAX8

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Validation Data Gallery

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  IF Staining of SKOV-3 using CL488-80756

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite® Plus 488 PAX8 antibody (CL488-80756, Clone: 6B14 ) at dilution of 1:400.

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Tested Applications

• Positive IF detected in: SKOV-3 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-80756 targets PAX8 in IF applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PAX8 fusion protein Ag0306
• Full Name: paired box 8
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa, 58 kDa
• GenBank Accession Number: BC001060
• Gene Symbol: PAX8
• Gene ID (NCBI): 7849
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinoma.