CoraLite®594-conjugated Profilin 1 Monoclonal antibody

Profilin 1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3A12E8

Cat no : CL594-67390

Synonyms

PFN1, profilin 1, Profilin I

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Validation Data Gallery

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  IF Staining of HeLa using CL594-67390

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 Profilin 1 antibody (CL594-67390, Clone: 3A12E8 ) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67390 targets Profilin 1 in IF applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Profilin 1 fusion protein Ag29206
• Full Name: profilin 1
• Calculated Molecular Weight: 140 aa, 15 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC006768
• Gene Symbol: PFN1
• Gene ID (NCBI): 5216
• RRID: AB_2920125
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Profilin-1 (PFN1) plays an important role in the control of actin dynamics, and could represent an important therapeutic target in several diseases. PFN1 is identified as a huntingtin aggregation inhibitor, and may serves as a tumor-suppressor. PFN1 is crucial for the conversion of monomeric (G)-actin to filamentous (F)-actin. Amyotrophic lateral sclerosis (ALS) is a late-onset neurodegenerative disorder resulting from motor neuron death. Cells expressing PFN1 mutants contain ubiquitinated, insoluble aggregates that in many cases contain the ALS-associated protein TDP-43. Recently, PFN1 is a potential biomarker for bladder cancer aggressiveness and may be of great clinical importance.