Profilin 1 Monoclonal antibody

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  WB analysis using 67390-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67390-1-Ig (Profilin 1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 67390-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67390-1-Ig (Profilin 1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67390-1-Ig

  WB result of Profilin 1 antibody (67390-1-Ig; 1:160000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Profilin 1 transfected HeLa cells.

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  WB analysis of HAP1 using 67390-1-Ig

  HAP1 (WT and PFN1 KO) lysates prepared with NP-40 buffer, 50 μg protein loaded. 67390-1-lg  incubated at 1:30,000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human colon cancer using 67390-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67390-1-Ig (Profilin 1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67390-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Profilin 1 antibody (67390-1-Ig, Clone: 3A12E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HAP1 using 67390-1-Ig

  HAP1 WT cells (green outline) and PFN1 KO cells (magenta outline) labelled with a green or a far-red fluorescence dye, respectively. Cells with 4% PFA and stained with 67390-1-lg at 1.0 μg/ml plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• Featured Product
• KD/KO Validated

Profilin 1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67390-1-Ig

Clone No.3A12E8

Publications(4)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PFN1, 3A12E8, ALS18, PFN 1, Profilin I

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Tested Applications

• Positive WB detected in: LNCaP cells, HAP1, HSC-T6 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, Neuro-2a cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HAP1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

67390-1-Ig targets Profilin 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Profilin 1 fusion protein Ag29206
• Full Name: profilin 1
• Calculated Molecular Weight: 140 aa, 15 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC006768
• Gene Symbol: Profilin 1
• Gene ID (NCBI): 5216
• RRID: AB_2882634
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07737
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Profilin-1 (PFN1) plays an important role in the control of actin dynamics, and could represent an important therapeutic target in several diseases. PFN1 is identified as a huntingtin aggregation inhibitor, and may serves as a tumor-suppressor. PFN1 is crucial for the conversion of monomeric (G)-actin to filamentous (F)-actin. Amyotrophic lateral sclerosis (ALS) is a late-onset neurodegenerative disorder resulting from motor neuron death. Cells expressing PFN1 mutants contain ubiquitinated, insoluble aggregates that in many cases contain the ALS-associated protein TDP-43. Recently, PFN1 is a potential biomarker for bladder cancer aggressiveness and may be of great clinical importance.

Publications

Species	Application	Title
human	IF	Cell Prolif; An artificial intelligence network-guided signature for predicting outcome and immunotherapy response in lung adenocarcinoma patients based on 26 machine learning algorithms; Authors - Nan Zhang
human	WB,IF	iScience; Methods to separate nuclear soluble fractions reflecting localizations in living cells; Authors - Yutaka Ogawa
human	WB	Diabetes Metab Syndr Obes; Profilin1 Promotes Renal Tubular Epithelial Cell Apoptosis in Diabetic Nephropathy Through the Hedgehog Signaling Pathway; Authors - Liping Mai
human	WB,IP,IF	F1000Res; The identification of high-performing antibodies for Profilin-1 for use in Western blot, immunoprecipitation and immunofluorescence; Authors - Riham Ayoubi ; KO Validated