Profilin 1 Monoclonal antibody

• Featured Product
• KD/KO Validated

Profilin 1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

3A12E8

Cat no : 67390-1-Ig

Synonyms

PFN1, profilin 1, Profilin I

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Validation Data Gallery

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  WB analysis using 67390-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67390-1-Ig (Profilin 1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 67390-1-Ig

  HAP1 (WT and PFN1 KO) lysates prepared with NP-40 buffer, 50 μg protein loaded. 67390-1-lg  incubated at 1:30,000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of HeLa using 67390-1-Ig

  WB result of Profilin 1 antibody (67390-1-Ig; 1:160000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Profilin 1 transfected HeLa cells.

• 

  WB analysis using 67390-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67390-1-Ig (Profilin 1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HAP1 using 67390-1-Ig

  HAP1 WT cells (green outline) and PFN1 KO cells (magenta outline) labelled with a green or a far-red fluorescence dye, respectively. Cells with 4% PFA and stained with 67390-1-lg at 1.0 μg/ml plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IF Staining of HeLa using 67390-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Profilin 1 antibody (67390-1-Ig, Clone: 3A12E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HSC-T6 cells, HAP1 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, Neuro-2a cells
• Positive IF detected in: HeLa cells, HAP1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

67390-1-Ig targets Profilin 1 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Profilin 1 fusion protein Ag29206
• Full Name: profilin 1
• Calculated molecular weight: 140 aa, 15 kDa
• Observed molecular weight: 13 kDa
• GenBank accession number: BC006768
• Gene symbol: PFN1
• Gene ID (NCBI): 5216
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Profilin-1 (PFN1) plays an important role in the control of actin dynamics, and could represent an important therapeutic target in several diseases. PFN1 is identified as a huntingtin aggregation inhibitor, and may serves as a tumor-suppressor. PFN1 is crucial for the conversion of monomeric (G)-actin to filamentous (F)-actin. Amyotrophic lateral sclerosis (ALS) is a late-onset neurodegenerative disorder resulting from motor neuron death. Cells expressing PFN1 mutants contain ubiquitinated, insoluble aggregates that in many cases contain the ALS-associated protein TDP-43. Recently, PFN1 is a potential biomarker for bladder cancer aggressiveness and may be of great clinical importance.

Publications

Species	Application	Title
human	IF	Cell Prolif; An artificial intelligence network-guided signature for predicting outcome and immunotherapy response in lung adenocarcinoma patients based on 26 machine learning algorithms; Authors - Nan Zhang
human	WB,IF	iScience; Methods to separate nuclear soluble fractions reflecting localizations in living cells; Authors - Yutaka Ogawa