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  • KD/KO Validated

Profilin 1 Monoclonal antibody

Profilin 1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3A12E8

Cat no : 67390-1-Ig

Synonyms

PFN1, profilin 1, Profilin I



Tested Applications

Positive WB detected inLNCaP cells, HAP1, HSC-T6 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, Neuro-2a cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HAP1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

67390-1-Ig targets Profilin 1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Profilin 1 fusion protein Ag29206
Full Name profilin 1
Calculated molecular weight 140 aa, 15 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC006768
Gene symbol PFN1
Gene ID (NCBI) 5216
RRIDAB_2882634
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Profilin-1 (PFN1) plays an important role in the control of actin dynamics, and could represent an important therapeutic target in several diseases. PFN1 is identified as a huntingtin aggregation inhibitor, and may serves as a tumor-suppressor. PFN1 is crucial for the conversion of monomeric (G)-actin to filamentous (F)-actin. Amyotrophic lateral sclerosis (ALS) is a late-onset neurodegenerative disorder resulting from motor neuron death. Cells expressing PFN1 mutants contain ubiquitinated, insoluble aggregates that in many cases contain the ALS-associated protein TDP-43. Recently, PFN1 is a potential biomarker for bladder cancer aggressiveness and may be of great clinical importance.

Protocols

Product Specific Protocols
WB protocol for Profilin 1 antibody 67390-1-IgDownload protocol
IHC protocol for Profilin 1 antibody 67390-1-IgDownload protocol
IF protocol for Profilin 1 antibody 67390-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Prolif

An artificial intelligence network-guided signature for predicting outcome and immunotherapy response in lung adenocarcinoma patients based on 26 machine learning algorithms

Authors - Nan Zhang
humanWB,IF

iScience

Methods to separate nuclear soluble fractions reflecting localizations in living cells

Authors - Yutaka Ogawa
humanWB

Diabetes Metab Syndr Obes

Profilin1 Promotes Renal Tubular Epithelial Cell Apoptosis in Diabetic Nephropathy Through the Hedgehog Signaling Pathway

Authors - Liping Mai
humanWB,IF,IP

F1000Res

The identification of high-performing antibodies for Profilin-1 for use in Western blot, immunoprecipitation and immunofluorescence

Authors - Riham Ayoubi
  • KO Validated