PFN1 Monoclonal antibody

PFN1 Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

CloneNo.

3A12E8

Cat no : 67390-1-Ig

Synonyms

PFN1, profilin 1, Profilin I



Tested Applications

Positive WB detected inHSC-T6 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, Neuro-2a cells
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67390-1-Ig targets PFN1 in WB, IF,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PFN1 fusion protein Ag29206
Full Name profilin 1
Calculated molecular weight 140 aa, 15 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC006768
Gene symbol PFN1
Gene ID (NCBI) 5216
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Profilin-1 (PFN1) plays an important role in the control of actin dynamics, and could represent an important therapeutic target in several diseases. PFN1 is identified as a huntingtin aggregation inhibitor, and may serves as a tumor-suppressor. PFN1 is crucial for the conversion of monomeric (G)-actin to filamentous (F)-actin. Amyotrophic lateral sclerosis (ALS) is a late-onset neurodegenerative disorder resulting from motor neuron death. Cells expressing PFN1 mutants contain ubiquitinated, insoluble aggregates that in many cases contain the ALS-associated protein TDP-43. Recently, PFN1 is a potential biomarker for bladder cancer aggressiveness and may be of great clinical importance.

Protocols

Product Specific Protocols
WB protocol for PFN1 antibody 67390-1-IgDownload protocol
IF protocol for PFN1 antibody 67390-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols