|Positive WB detected in||HeLa cells, MCF-7 cells, COLO 320 cells|
|Positive IP detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
15956-1-AP targets PLK2 in WB, IP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PLK2 fusion protein Ag8710|
|Full Name||polo-like kinase 2 (Drosophila)|
|Calculated molecular weight||685 aa, 78 kDa|
|Observed molecular weight||75-80 kDa|
|GenBank accession number||BC013879|
|Gene ID (NCBI)||10769|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PLK2(Polo-like kinase 2) is also named as SNK and belongs to the Ser/Thr protein kinase family. It plays a role in normal cell division. Plk2 expression is detected only in G1, and endogenous Plk2 is rapidly turned over. Therefore, the expression of the three mammalian Plks during the cell cycle resembles the successive transition of the cyclins, prompting speculation that Plks may reg-ulate the cell cycle in a manner analogous to that of Cdks(PMID:12972611).
J Biochem Mol Toxicol
PLK2 protects retinal ganglion cells from oxidative stress by potentiating Nrf2 signaling via GSK-3β.
MicroRNA-27b up-regulated by human papillomavirus 16 E7 promotes proliferation and suppresses apoptosis by targeting polo-like kinase2 in cervical cancer.