POLA2 Recombinant monoclonal antibody

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  WB analysis using 86856-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86856-3-RR (POLA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86856-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86856-3-RR against Human POLA2 were performed. The affinity constant is below 1 pM.

POLA2 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86856-3-RR

Clone No.251924F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DNA polymerase alpha subunit B

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, COLO 320 cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells, PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86856-3-RR targets POLA2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0271

  Product name: Recombinant human POLA2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 17-275 aa of BC001347

  Sequence: LDCEEALIEKLVELCVQYGQNEEGMVGELIAFCTSTHKVGLTSEILNSFEHEFLSKRLSKARHSTCKDSGHAGARDIVSIQELIEVEEEEEILLNSYTTPSKGSQKRAISTPETPLTKRSVSTRSPHQLLSPSSFSPSATPSQKYNSRSNRGEVVTSFGLAQGVSWSGRGGAGNISLKVLGCPEALTGSYKSMFQKLPDIREVLTCKIEELGSELKEHYKIEAFTPLLAPAQEPVTLLGQIGCDSNGKLNNKSVILEGD

• Full Name: polymerase (DNA directed), alpha 2 (70kD subunit)
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC001347
• Gene Symbol: POLA2
• Gene ID (NCBI): 23649
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14181
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.