POLR2F Monoclonal antibody

POLR2F Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D4D12

Cat no : 68511-1-Ig

Synonyms

HRBP14.4, POLR2F, POLRF, RPABC14.4, RPABC2, RPB14.4, RPB6, RPB6 homolog, RPC15

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Validation Data Gallery

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  WB analysis using 68511-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68511-1-Ig (POLR2F antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68511-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68511-1-Ig (POLR2F antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LNCaP cells, NIH/3T3 cells, MCF-7 cells, A549 cells, HeLa cells, 4T1 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68511-1-Ig targets POLR2F in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: POLR2F fusion protein Ag7472
• Full Name: polymerase (RNA) II (DNA directed) polypeptide F
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC003582
• Gene Symbol: POLR2F
• Gene ID (NCBI): 5435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.