Pancreatic Polypeptide Polyclonal antibody

Pancreatic Polypeptide Polyclonal Antibody for IHC, IF-P, FC (Intra), ELISA

Cat No. 15493-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, FC (Intra), ELISA

PPY, PH, Pancreatic prohormone, Pancreatic polypeptide Y, Pancreatic polypeptide prohormone

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat pancreas tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15493-1-AP targets Pancreatic Polypeptide in IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7886

Product name: Recombinant human PPY protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-95 aa of BC032225

Sequence: MAAARLCLSLLLLSTCVALLLQPLLGAQGAPLEPVYPGDNATPEQMAQYAADLRRYINMLTRPRYGKRHKEDTLAFSEWGSPHAAVPRELSPLDL

Predict reactive species
Full Name pancreatic polypeptide
Calculated Molecular Weight 10 kDa
GenBank Accession NumberBC032225
Gene Symbol Pancreatic Polypeptide
Gene ID (NCBI) 5539
RRIDAB_2878145
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP01298
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for Pancreatic Polypeptide antibody 15493-1-APDownload protocol
IF protocol for Pancreatic Polypeptide antibody 15493-1-APDownload protocol
FC protocol for Pancreatic Polypeptide antibody 15493-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Transpl Int

Formation of Re-Aggregated Neonatal Porcine Islet Clusters Improves In Vitro Function and Transplantation Outcome

Authors - M Honarpisheh
IF

Vet Med Sci

Histology and immunofluorescent study of the pancreas in lovebird (Agapornis personatus)

Authors - Nader Goodarzi
mouseIF

Diabetes

Mettl3-Mediated m6A Methylation Controls Pancreatic Bipotent Progenitor Fate and Islet Formation

Authors - Jiajun Sun

Cell Rep

A ONECUT1 regulatory, non-coding region in pancreatic development and diabetes

Authors - Sarah Merz
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