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CoraLite®650-conjugated PTEN Polyclonal antibody

PTEN Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite®650 Fluorescent Dye

Cat no : CL650-22034

Synonyms

10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1



Tested Applications

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 22034-1-AP

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL650-22034 is PTEN Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTEN fusion protein Ag17274
Full Name phosphatase and tensin homolog
Calculated molecular weight 47 kDa
Observed molecular weight 55 kDa, 68 kDa
GenBank accession numberBC005821
Gene symbol PTEN
Gene ID (NCBI) 5728
Conjugate CoraLite®650 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Phosphatase and tensin homolog (PTEN) is an enzyme key in cell cycle regulation and in tumor suppression.


What is the molecular weight of PTEN?

PTEN is composed of 403 amino acid residues with an N-terminal domain with phosphatase activity and a 

C-terminal C2 domain that binds phospholipid membranes (PMID:14749127). This antibody can recognize 

two isoforms with MWs of 55kDa and 68 kDa.


What is the function of PTEN?

Widely expressed throughout the body, PTEN is found in the cytoplasm and inhibits cell cycle progression 

through phosphatase activity. It regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate (PIP3) 

by catalyzing the dephosphorylation of the inositol ring. This function counteracts the growth-stimulating 

activity of the PI3K/AKT signaling pathway and thereby suppresses proliferation. Therefore, in homeostasis, 

PTEN promotes chromosome stability and DNA repair (PMID: 24387334).Expression and function can be 

regulated by posttranslational modifications, including acetylation for decreased catalytic activity and 

ubiquitination for protein degradation, nuclear localization, and inhibition of phosphatase activity 

(PMID: 24656806).


What is the role of PTEN in cancer?

PTEN was first identified as a tumor suppressor when mutations or deletions were identified by two separate 

groups in a number of different cancer types including gliomas, breast, prostate, and kidney cancer 

(PMID: 9090379; PMID: 9072974). Non-inherited, or somatic, mutations in PTEN that lead to a loss of function 

in the protein have been shown to drive malignancy in tumors. PTEN hamartoma tumor syndrome (PHTS) is a 

group of inherited disorders that occur as a result of autosomal dominant mutations in the PTEN gene. These 

disorders include Cowden syndrome, Bannayan-Riley-Ruvalcaba syndrome, and Proteus syndrome. Because of 

the constitutive expression of PTEN, the symptoms are varied but often include neurodevelopmental issues and 

an increased risk of developing tumors, both benign and malignant (PMID: 26564076).


Protocols

Product Specific Protocols
FC protocol for CL650 PTEN antibody CL650-22034Download protocol
Standard Protocols
Click here to view our Standard Protocols