Phospho-Histone H3 (Ser28) Recombinant monoclonal antibody

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  WB analysis of HeLa using 82828-15-RR

  Nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 82828-15-RR (Phospho-Histone H3 (Ser28) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control.

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  ChIP experiment of HeLa using 82828-15-RR

  Chromatin was prepared from HeLa cells treated with Nocodazole (100 ng/ml) for 16 h. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of Phospho-Histone H3 (Ser28) (82828-15-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

Phospho-Histone H3 (Ser28) Uni-rAb^® Recombinant Antibody for WB, ELISA, ChIP-qPCR

Cat No. 82828-15-RR

Clone No.250369A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA, ChIP-qPCR

Synonyms

H3, Histone H3 (Ser28), p Histone H3, p Histone H3 (Ser28), p Histone H3 Ser28

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Tested Applications

• Positive WB detected in: nocodazole treated HeLa cells
• Positive ChIP-qPCR detected in: Nocodazole-HeLa (100 ng/ml, 16 h) HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• CHIP-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82828-15-RR targets Phospho-Histone H3 (Ser28) in WB, ELISA, ChIP-qPCR applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P68431
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone modifications and variants have key roles in the activation and silencing of genes. Phosphorylation of histone H3 at serine 10 and serine 28 is involved in transcriptional activation of genes responding to stress or mitogen-stimulated signaling pathways. Histone H3 phosphorylated at serine 28 was located at the promoter region of the transcriptionally active, but not competent, histone H5 and β-globin genes (PMID: 17913747).