Phospho-PKA C (Thr198) Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of HeLa using 87365-3-RR (same clone as 87365-3-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 87365-3-RR (Phospho-PKA C (Thr198) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87365-3-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 87365-3-RR (same clone as 87365-3-PBS)

  Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87365-3-RR (Phospho-PKA C (Thr198) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87365-3-PBS in a different storage buffer formulation.

Phospho-PKA C (Thr198) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87365-3-PBS

Clone No.252621G1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Phospho-PKA C (Thr197), Phos-PKA C, PKA C-beta, PKA C-gamma, P-PKA C

---

Product Information

87365-3-PBS targets Phospho-PKA C (Thr198) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: protein kinase, cAMP-dependent, catalytic, alpha
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 38-43 kDa
• GenBank Accession Number: NM_002730
• Gene Symbol: PRKACA
• Gene ID (NCBI): 5566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P17612
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Protein kinase A (PKA) is a 3′-5′-cyclic adenosine monophosphate (cAMP)-dependent kinase that is at the center of diverse biological functions in numerous systems. Inactive PKA is composed of two catalytic (PKA-C) and two regulatory (PKA-R) subunits. The binding of cAMP to the PKA-R subunits activates the enzyme, causing the release of PKA-C. Humans have three genes encoding PKA-C: PRKACA, PRKACB, and PRKACG, and two types of PKA-R's: type PKA-RI (PKA-Riα and PKA-Riβ) and type PKA-RII (PKA-RIIα and PKA-RIIβ).(PMID: 36431013, PMID: 39592424)
This antibody recognizes the phosphorylation at the Thr198 site of PKA-C (including PRKACA, PRKACB, and PRKACG). After removing methionine, the site is also called Thr197.