Phospho-PKA C (Thr198) Recombinant monoclonal antibody

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• Phospho-PKA C (Thr198) Recombinant monoclonal antibody (87365-3-RR)

Phospho-PKA C (Thr198) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 87365-3-RR

Clone No.252621G1

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

Phospho-PKA C (Thr197), Phos-PKA C, PKA C-beta, PKA C-gamma, P-PKA C

• 

  WB analysis of HeLa using 87365-3-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 87365-3-RR (Phospho-PKA C (Thr198) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 87365-3-RR

  Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87365-3-RR (Phospho-PKA C (Thr198) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HeLa using 87365-3-RR

  1X10^6 HeLs cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-PKA C (Thr198) Recombinant monoclonal antibody (87365-3-RR, Clone:252621G1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HeLa cells
• Positive FC (Intra) detected in: λ phosphatase treated HeLa cells

This antibody recognizes the phosphorylation at the Thr198 site of PKA-C (including PRKACA, PRKACB, and PRKACG).

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87365-3-RR targets Phospho-PKA C (Thr198) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: protein kinase, cAMP-dependent, catalytic, alpha
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 38-43 kDa
• GenBank Accession Number: NM_002730
• Gene Symbol: PRKACA
• Gene ID (NCBI): 5566
• RRID: AB_3745487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P17612
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein kinase A (PKA) is a 3′-5′-cyclic adenosine monophosphate (cAMP)-dependent kinase that is at the center of diverse biological functions in numerous systems. Inactive PKA is composed of two catalytic (PKA-C) and two regulatory (PKA-R) subunits. The binding of cAMP to the PKA-R subunits activates the enzyme, causing the release of PKA-C. Humans have three genes encoding PKA-C: PRKACA, PRKACB, and PRKACG, and two types of PKA-R's: type PKA-RI (PKA-Riα and PKA-Riβ) and type PKA-RII (PKA-RIIα and PKA-RIIβ).(PMID: 36431013, PMID: 39592424)
This antibody recognizes the phosphorylation at the Thr198 site of PKA-C (including PRKACA, PRKACB, and PRKACG). After removing methionine, the site is also called Thr197.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Phytomedicine; Differential and complementary effects of Baizhu and Fuling on spleen deficiency syndrome by regulating microbiota-gut-metabolite axis.; Authors - Yubin Xu
mouse	WB	Int Immunopharmacol; Melatonin orchestrates cAMP-PKA signaling to alleviate zearalenone-impaired the estradiol synthesis in mammal ovary.; Authors - Qiao Li