CoraLite® Plus 594-conjugated RANGAP1 Monoclonal antibody

RANGAP1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

1F3A4

Cat no : CL594-67146

Synonyms

Fug1, KIAA1835, RANGAP1, SD

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-67146

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 594 RANGAP1 antibody (CL594-67146, Clone: 1F3A4 ) at dilution of 1:1000.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67146 targets RANGAP1 in IF applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RANGAP1 fusion protein Ag26136
• Full Name: Ran GTPase activating protein 1
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 64 kDa, 82 kDa
• GenBank Accession Number: BC014044
• Gene Symbol: RANGAP1
• Gene ID (NCBI): 5905
• Conjugate: CoraLite® Plus 594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 594 nm / 615 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RanGAP1 is a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. In mammalian cells, unmodified RanGAP1 is predominantly cytoplasmic, whereas modification by small ubiquitin-related modifier protein (SUMO) targets RanGAP1 to the cytoplasmic filaments of nuclear pore complex (NPC) where it forms a complex with RanBP2 and Ubc9. Phosphorylation of RanGAP1 occurs in a cell-cycle-dependent manner and may play a role in regulating RanGAP1 catalytic activity. We got 64 kDa (cytoplasmic Ran GAP1) and 82 kDa (SUMO-1 modified Ran GAP1) in western blotting.