RANGAP1 Monoclonal antibody

RANGAP1 Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1F3A4

Cat no : 67146-1-Ig

Synonyms

Fug1, KIAA1835, RANGAP1, SD



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunofluorescence (IF)IF : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

67146-1-Ig targets RANGAP1 in WB, IF,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RANGAP1 fusion protein Ag26136
Full Name Ran GTPase activating protein 1
Calculated molecular weight 64 kDa
Observed molecular weight 64 kDa, 82 kDa
GenBank accession numberBC014044
Gene symbol RANGAP1
Gene ID (NCBI) 5905
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

RanGAP1 is a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. In mammalian cells, unmodified RanGAP1 is predominantly cytoplasmic, whereas modification by small ubiquitin-related modifier protein (SUMO) targets RanGAP1 to the cytoplasmic filaments of nuclear pore complex (NPC) where it forms a complex with RanBP2 and Ubc9. Phosphorylation of RanGAP1 occurs in a cell-cycle-dependent manner and may play a role in regulating RanGAP1 catalytic activity. We got 64 kDa (cytoplasmic Ran GAP1) and 82 kDa (SUMO-1 modified Ran GAP1) in western blotting.

Protocols

Product Specific Protocols
WB protocol for RANGAP1 antibody 67146-1-IgDownload protocol
IF protocol for RANGAP1 antibody 67146-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols