RBP7 Polyclonal antibody

RBP7 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14541-1-AP

Synonyms

CRBP4, CRABP-IV, CRABP4, CRABP IV, Cellular retinoic acid-binding protein IV

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Validation Data Gallery

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  WB analysis of HEK-293 using 14541-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14541-1-AP (RBP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse adipose using 14541-1-AP

  mouse adipose tissue were subjected to SDS PAGE followed by western blot with 14541-1-AP (RBP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14541-1-AP

  IP result of anti-RBP7 (IP:14541-1-AP, 4ug; Detection:14541-1-AP 1:500) with HEK-293 cells lysate 3000ug.

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  IHC staining of human colon cancer using 14541-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14541-1-AP (RBP7 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 14541-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14541-1-AP (RBP7 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 14541-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14541-1-AP (RBP7 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14541-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14541-1-AP (RBP7 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of human kidney using 14541-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using RBP7 antibody (14541-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human colon cancer using 14541-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using RBP7 antibody (14541-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 14541-1-AP

  Immunofluorescent analysis of HEK-293 cells using 14541-1-AP (RBP7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse adipose tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human kidney tissue, human colon cancer tissue
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14541-1-AP targets RBP7 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBP7 fusion protein Ag6045
• Full Name: retinol binding protein 7, cellular
• Calculated Molecular Weight: 15.5 kDa
• Observed Molecular Weight: 15-18 kDa
• GenBank Accession Number: BC063013
• Gene Symbol: RBP7
• Gene ID (NCBI): 116362
• RRID: AB_2179293
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cancer Cell Int; RBP7 functions as a tumor suppressor in HR + breast cancer by inhibiting the AKT/SREBP1 pathway and reducing fatty acid; Authors - Yue Yu