ROCK1 Monoclonal antibody

ROCK1 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

3G5E12

Cat no : 66782-1-Ig

Synonyms

p160 ROCK 1, P160ROCK, PRO0435, ROCK1



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

66782-1-Ig targets ROCK1 in WB,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ROCK1 fusion protein Ag16317
Full Name Rho-associated, coiled-coil containing protein kinase 1
Calculated molecular weight 1354 aa, 158 kDa
Observed molecular weight 150-160 kDa
GenBank accession numberBC113114
Gene symbol ROCK1
Gene ID (NCBI) 6093
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.

Protocols

Product Specific Protocols
WB protocol for ROCK1 antibody 66782-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Am J Transl Res

β-catenin regulates myocardial ischemia/reperfusion injury following heterotopic heart transplantation in mice by modulating PTEN pathways.

Authors - Qian Ban
humanWB

J Cell Biochem

Lnc-MUC20-9 binds to ROCK1 and functions as a tumor suppressor in bladder cancer.

Authors - Ranran Dai