|Positive WB detected in||HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Sample-dependent, check data in validation data gallery|
66782-1-Ig targets ROCK1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
|Tested Reactivity||Human, Mouse, Rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||ROCK1 fusion protein Ag16317|
|Full Name||Rho-associated, coiled-coil containing protein kinase 1|
|Calculated molecular weight||1354 aa, 158 kDa|
|Observed molecular weight||150-160 kDa|
|GenBank accession number||BC113114|
|Gene ID (NCBI)||6093|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.
J Cell Biochem
Lnc-MUC20-9 binds to ROCK1 and functions as a tumor suppressor in bladder cancer.
Am J Transl Res
β-catenin regulates myocardial ischemia/reperfusion injury following heterotopic heart transplantation in mice by modulating PTEN pathways.
Strains producing different short-chain fatty acids alleviate DSS-induced ulcerative colitis by regulating intestinal microecology
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Balawant (Verified Customer) (12-20-2022)
I have used this antibody for western blot and it is working great