Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66782-1-Ig targets ROCK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16317 Product name: Recombinant human ROCK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 401-710 aa of BC113114 Sequence: SNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKNQLEDLKKVSQNSQLANEKLSQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTDKDYYQLQAILEAERRDRGHDSEMIGDLQARITSLQEEVKHLKHNLEKVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSLQQRLEQEVNEHKVTKARLTDKHQSIEEAKS Predict reactive species |
| Full Name | Rho-associated, coiled-coil containing protein kinase 1 |
| Calculated Molecular Weight | 1354 aa, 158 kDa |
| Observed Molecular Weight | 150-160 kDa |
| GenBank Accession Number | BC113114 |
| Gene Symbol | ROCK1 |
| Gene ID (NCBI) | 6093 |
| RRID | AB_2882127 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q13464 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ROCK1 antibody 66782-1-Ig | Download protocol |
| WB protocol for ROCK1 antibody 66782-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ROCK1 Monoclonal antibody (3G5E12), catalog number 66782-1-Ig, has 5 total citations. These appear in Food & Function, Animals (Basel), Journal of Cellular Biochemistry, Gene, and American Journal of Translational Research. The associated research fields include intestinal microecology and ulcerative colitis, viral replication (Peste des Petits Ruminants), bladder cancer, renal cell carcinoma, and myocardial ischemia/reperfusion injury in heart transplantation.
| Species | Application | Title |
|---|---|---|
Food Funct Strains producing different short-chain fatty acids alleviate DSS-induced ulcerative colitis by regulating intestinal microecology | ||
Animals (Basel) Peste Des Petits Ruminants Virus Infection Induces Syncytium Formation via RhoA-Rock1 Signaling Pathway to Promote Viral Replication. | ||
J Cell Biochem Lnc-MUC20-9 binds to ROCK1 and functions as a tumor suppressor in bladder cancer. | ||
Gene LINC00645 inhibits renal cell carcinoma progression by interacting with HNRNPA2B1 to regulate the ROCK1 mRNA stability | ||
Am J Transl Res β-catenin regulates myocardial ischemia/reperfusion injury following heterotopic heart transplantation in mice by modulating PTEN pathways. |
Reviews
What customers say
All four reviewers gave this antibody a perfect 5-star rating. Users consistently report excellent Western Blot performance, with one noting "very sharp bands" at 1:1000 dilution and another confirming strong results at 1:2000. The antibody was successfully used across multiple applications including Western Blot and Immunofluorescence, and tested on various cell types such as colon cancer cells, CRC cells, and renal cancer cell lines (CAKI-2). Overall, customers describe it as a reliable, high-performing reagent that works great out of the box.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Iram (Verified Customer) (03-23-2026) | It gives very sharp bands.
|
Sai Sindhura (Verified Customer) (03-23-2026) | good antibody
|
Iram (Verified Customer) (09-18-2025) | Very good antibody
|
Balawant (Verified Customer) (12-20-2022) | I have used this antibody for western blot and it is working great
|
