RXRA Monoclonal antibody

RXRA Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

4H6C4

Cat no : 60198-1-Ig

Synonyms

NR2B1, Retinoid X receptor alpha, retinoid X receptor, alpha, RXRA



Tested Applications

Positive WB detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60198-1-Ig is RXRA Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RXRA fusion protein Ag0987
Full Name retinoid X receptor, alpha
Calculated molecular weight 462 aa, 51 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC007925
Gene symbol RXRA
Gene ID (NCBI) 6256
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Retinoid X receptor alpha (RXRA). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.

Protocols

Product Specific Protocols
WB protocol for RXRA antibody 60198-1-IgDownload protocol
Standard Protocols
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