Validation Data Gallery
|Positive WB detected in||HeLa cells, A549 cells|
|Positive IP detected in||A549 cells|
|Positive IHC detected in||human pancreas cancer tissue, human liver cancer tissue, human colon cancer tissue, human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10245-1-AP targets S100A6 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||S100A6 fusion protein Ag0385|
|Full Name||S100 calcium binding protein A6|
|Calculated molecular weight||10 kDa|
|Observed molecular weight||10 kDa|
|GenBank accession number||BC001431|
|Gene ID (NCBI)||6277|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis. The antibody is specific for S100A6.
|Product Specific Protocols|
|WB protocol for S100A6 antibody 10245-1-AP||Download protocol|
|IHC protocol for S100A6 antibody 10245-1-AP||Download protocol|
|IF protocol for S100A6 antibody 10245-1-AP||Download protocol|
|IP protocol for S100A6 antibody 10245-1-AP||Download protocol|
|FC protocol for S100A6 antibody 10245-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Br J Cancer
ZEB1 and IL-6/11-STAT3 signalling cooperate to define invasive potential of pancreatic cancer cells via differential regulation of the expression of S100 proteins.
J Toxicol Environ Health A
Radon-induced proteomic profile of lung tissue in rats.
J Toxicol Environ Health A
Proteomic analysis of lung tissue of rats exposed to cigarette smoke and radon.
Dig Dis Sci
S100A6 as a Potential Serum Prognostic Biomarker and Therapeutic Target in Gastric Cancer.
Mol Reprod Dev
Protein profiling the differences between diabetic and normal mouse cumulus cells.
S100 proteins regulate the interaction of Hsp90 with Cyclophilin 40 and FKBP52 through their tetratricopeptide repeats.