Validation Data Gallery
|Positive WB detected in||A549 cells, HeLa cells|
|Positive IP detected in||A549 cells|
|Positive IHC detected in||human stomach cancer tissue, human liver cancer tissue, human pancreas cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:400-1:1600|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
10245-1-AP targets S100A6 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||S100A6 fusion protein Ag0385|
|Full Name||S100 calcium binding protein A6|
|Calculated molecular weight||10 kDa|
|Observed molecular weight||10 kDa|
|GenBank accession number||BC001431|
|Gene ID (NCBI)||6277|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis. The antibody is specific for S100A6.
|Product Specific Protocols|
|WB protocol for S100A6 antibody 10245-1-AP||Download protocol|
|IHC protocol for S100A6 antibody 10245-1-AP||Download protocol|
|IF protocol for S100A6 antibody 10245-1-AP||Download protocol|
|IP protocol for S100A6 antibody 10245-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cellular architecture of human brain metastases.
Nat Cell Biol
Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.
Sci Transl Med
Mutations in the vesicular trafficking protein annexin A11 are associated with amyotrophic lateral sclerosis.
Cancer Commun (Lond)
Candidate therapeutic agents in a newly established triple wild-type mucosal melanoma cell line.
S100A6 promotes cell proliferation in human nasopharyngeal carcinoma via the p38/MAPK signalling pathway.
Genetic screening of ANXA11 revealed novel mutations linked to amyotrophic lateral sclerosis.