SAMD4B Polyclonal antibody

• Featured Product
• KD/KO Validated

SAMD4B Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17723-1-AP

Synonyms

hSmaug2, Protein Smaug homolog 2, SAMD4B, Smaug 2, SMAUG2, SMGB

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Validation Data Gallery

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  WB analysis of HeLa using 17723-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17723-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17723-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17723-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 17723-1-AP

  Immunofluorescent analysis of HepG2 cells, using SAMD4B antibody 17723-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

17723-1-AP targets SAMD4B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAMD4B fusion protein Ag12113
• Full Name: sterile alpha motif domain containing 4B
• Calculated Molecular Weight: 694 aa, 75 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC054518
• Gene Symbol: SAMD4B
• Gene ID (NCBI): 55095
• RRID: AB_2183381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Mol Immunol; SAMD4 family members suppress human hepatitis B virus by directly binding to the Smaug recognition region of viral RNA.; Authors - Yuze Wang ; KO Validated
human	WB,IF	Mol Med Rep; miR‑451 suppresses the malignant characteristics of colorectal cancer via targeting SAMD4B.; Authors - Chunrong Wu