SENP5-Specific Polyclonal antibody, PBS Only

• 

  WB analysis of mouse liver using 19529-1-AP (same clone as 19529-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19529-1-AP (SENP5-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19529-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 19529-1-AP (same clone as 19529-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19529-1-AP (SENP5-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19529-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 19529-1-AP (same clone as 19529-1-PBS)

  IP result of anti-SENP5-Specific (IP:19529-1-AP, 4ug; Detection:19529-1-AP 1:300) with HeLa cells lysate 520ug. This data was developed using the same antibody clone with 19529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 19529-1-AP (same clone as 19529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 19529-1-AP (same clone as 19529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 19529-1-AP (same clone as 19529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 19529-1-AP (same clone as 19529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 19529-1-AP (same clone as 19529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 19529-1-AP (same clone as 19529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19529-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SENP5-Specific Polyclonal Antibody for IHC, IP, Indirect ELISA

Cat No. 19529-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, Indirect ELISA

Synonyms

SENP5, Sentrin-specific protease 5, Sentrin/SUMO-specific protease SENP5, EC:3.4.22.-

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Product Information

19529-1-PBS targets SENP5-Specific in IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: SUMO1/sentrin specific peptidase 5
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: NM_152699
• Gene Symbol: SENP5
• Gene ID (NCBI): 205564
• RRID: AB_10643377
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96HI0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SENP5, also named as FKSG45, belongs to the peptidase C48 family. SENP5 is a protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. SENP5 has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. It is required for cell division. The antibody is specific to SENP5.