SENP5-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

SENP5-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 19529-1-AP

Synonyms

SENP5, SENP5-Specific, Sentrin specific protease 5

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Validation Data Gallery

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  WB analysis using 19529-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19529-1-AP (SENP5-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 19529-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19529-1-AP (SENP5-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 19529-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19529-1-AP (SENP5-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19529-1-AP

  IP result of anti-SENP5-Specific (IP:19529-1-AP, 4ug; Detection:19529-1-AP 1:300) with HeLa cells lysate 520ug.

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  IHC staining of human cervical cancer using 19529-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 19529-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 19529-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 19529-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 19529-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 19529-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human cervical cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

19529-1-AP targets SENP5-Specific in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SUMO1/sentrin specific peptidase 5
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: NM_152699
• Gene Symbol: SENP5
• Gene ID (NCBI): 205564
• RRID: AB_10643377
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SENP5, also named as FKSG45, belongs to the peptidase C48 family. SENP5 is a protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. SENP5 has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. It is required for cell division. The antibody is specific to SENP5.

Publications

Species	Application	Title
	WB	EMBO Rep; The SUMO protease SENP3 regulates mitochondrial autophagy mediated by Fis1.; Authors - Emily Waters
human	WB	J Cell Sci; SUMO proteases SENP3 and SENP5 spatiotemporally regulate the kinase activity of Aurora A.; Authors - Bin Yu
human	WB	J Cell Sci; SUMO proteases SENP3 and SENP5 spatiotemporally regulate the kinase activity of Aurora A.; Authors - Bin Yu
human,mouse	WB	iScience; Drp1 SUMO/deSUMOylation by Senp5 isoforms influences ER tubulation and mitochondrial dynamics to regulate brain development.; Authors - Seiya Yamada ; KD Validated
mouse	WB,IHC,IF	J Comp Neurol; Synaptic localization of the SUMOylation-regulating protease SENP5 in the adult mouse brain.; Authors - Hiroki Akiyama ; KD Validated
mouse	WB	Cell Rep; SENP7 deSUMOylase-governed transcriptional program coordinates sarcomere assembly and is targeted in muscle atrophy; Authors - Mamta Amrute-Nayak