SNAPIN Polyclonal antibody

SNAPIN Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 10055-1-AP

Synonyms

SNAP associated protein, SNAP25BP, SNAPAP, SNAPIN



Tested Applications

Positive WB detected inA375 cells, HEK-293 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman testis tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10055-1-AP targets SNAPIN in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNAPIN fusion protein Ag0101
Full Name SNAP-associated protein
Calculated molecular weight 15 kDa
Observed molecular weight 15-18 kDa
GenBank accession numberBC000761
Gene symbol SNAPIN
Gene ID (NCBI) 23557
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Snapin, a protein of relative molecular weight of 15kd, is implicated in neurotransmission by binding to SNAP-25. Snapin was enriched in neurons and exclusively located on synaptic vesicle membrane, which may be a PKA target for modulating transmitter release through the cAMP-dependent signal-transduction pathway. This anitbody can recognize the 15-18kd(Monomer) and 30-36kd(Dimer) forms of SNAPIN.

Protocols

Product Specific Protocols
WB protocol for SNAPIN antibody 10055-1-APDownload protocol
IHC protocol for SNAPIN antibody 10055-1-APDownload protocol
IF protocol for SNAPIN antibody 10055-1-APDownload protocol
IP protocol for SNAPIN antibody 10055-1-APDownload protocol
FC protocol for SNAPIN antibody 10055-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

J Thromb Haemost

Dysregulation of PLDN (pallidin) is a mechanism for platelet dense granule deficiency in RUNX1 haplodeficiency.

Authors - G F Mao
mouseWB

J Neuroendocrinol

PMID: 24118254

Authors - Makani Vishruti V
humanWB

Traffic

BORC regulates late endosomal/lysosomal size through PIKfyve-dependent phosphatidylinositol-3,5-bisphosphate.

Authors - Teodor E Yordanov
humanIHC

Skelet Muscle

A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome.

Authors - Blandin Gaëlle G
humanWB

Pigment Cell Melanoma Res

A BLOC-1 mutation screen reveals a novel BLOC1S3 mutation in Hermansky-Pudlak Syndrome type 8.

Authors - Cullinane Andrew R AR
humanWB

J Cell Biol

LAMTOR/Ragulator is a negative regulator of Arl8b- and BORC-dependent late endosomal positioning.

Authors - Przemyslaw A Filipek