|Positive WB detected in||A375 cells, HEK-293 cells, mouse brain tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human testis tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10055-1-AP targets SNAPIN in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SNAPIN fusion protein Ag0101|
|Full Name||SNAP-associated protein|
|Calculated molecular weight||15 kDa|
|Observed molecular weight||15-18 kDa|
|GenBank accession number||BC000761|
|Gene ID (NCBI)||23557|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Snapin, a protein of relative molecular weight of 15kd, is implicated in neurotransmission by binding to SNAP-25. Snapin was enriched in neurons and exclusively located on synaptic vesicle membrane, which may be a PKA target for modulating transmitter release through the cAMP-dependent signal-transduction pathway. This anitbody can recognize the 15-18kd(Monomer) and 30-36kd(Dimer) forms of SNAPIN.
|Product Specific Protocols|
|WB protocol for SNAPIN antibody 10055-1-AP||Download protocol|
|IHC protocol for SNAPIN antibody 10055-1-AP||Download protocol|
|IF protocol for SNAPIN antibody 10055-1-AP||Download protocol|
|IP protocol for SNAPIN antibody 10055-1-AP||Download protocol|
|FC protocol for SNAPIN antibody 10055-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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