Tested Applications
| Positive WB detected in | U2OS cells, HeLa cells, ROS1728 cells, RAW 264.7 cells, pig brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human liver cancer tissue, human liver tissue, human colon cancer tissue, human pancreas cancer tissue, mouse liver tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HUVEC cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66474-1-Ig targets SOD2 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, pig, goat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21388 Product name: Recombinant human SOD2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-140 aa of BC016934 Sequence: MLSRAVCGTSRQLAPALGYLGSRQKHSLPDLPYDYGALEPHINAQIMQLHHSKHHAAYVNNLNVTEEKYQEALAKGRFQAERREAVPGRGDPREPGPIRTGLSVEENSLRICTGSEFSRHDSLSFKHMVYLIVEGVPRWV Predict reactive species |
| Full Name | superoxide dismutase 2, mitochondrial |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC016934 |
| Gene Symbol | SOD2 |
| Gene ID (NCBI) | 6648 |
| RRID | AB_2881840 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04179 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for SOD2 antibody 66474-1-Ig | Download protocol |
| IF protocol for SOD2 antibody 66474-1-Ig | Download protocol |
| IHC protocol for SOD2 antibody 66474-1-Ig | Download protocol |
| WB protocol for SOD2 antibody 66474-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SOD2 Monoclonal antibody (3A6C2), catalog number 66474-1-Ig, has 91 citations appearing in journals including ACS Nano, Redox Biology, Advanced Science, Free Radical Biology and Medicine, Antioxidants, Stem Cell Research & Therapy, Scientific Reports, Cell Death & Disease, and Diabetes. Associated research fields span oxidative stress, mitochondrial dysfunction, ferroptosis, cardiomyopathy, neurodegeneration, cancer, diabetes, inflammation, wound healing, reproductive biology, and aging.
| Species | Application | Title |
|---|---|---|
ACS Nano Amplification of Metalloregulatory Proteins in Macrophages by Bioactive ZnMn@SF Hydrogels for Spinal Cord Injury Repair | ||
Metabolism Increased adrenal steroidogenesis and suppressed corticosteroid responsiveness in critical COVID-19 | ||
Redox Biol Inhibition of MST1 ameliorates neuronal apoptosis via GSK3β/β-TrCP/NRF2 pathway in spinal cord injury accompanied by diabetes | ||
Redox Biol Energy stress modulation of AMPK/FoxO3 signaling inhibits mitochondria-associated ferroptosis | ||
Cardiovasc Diabetol Cardiometabolic benefits of fenofibrate in heart failure related to obesity and diabetes |
Reviews
What customers say
The single review for 66474-1-Ig is a 5-star rating from a researcher who used the antibody for Western Blot on fallopian tube epithelial cells. They reported excellent performance at a 1:2000 dilution with overnight incubation, describing it as working "very well." Overall, the feedback is positive, highlighting reliable results under standard WB conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Iru (Verified Customer) (07-30-2020) | This antibody works very well at 1:2000 dilution with O/N incubation.
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