SOD2 Monoclonal antibody

SOD2 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

3A6C2

Cat no : 66474-1-Ig

Synonyms

IPO B, Mn SOD, MNSOD, SOD2, SODM



Tested Applications

Positive WB detected inROS1728 cells, HeLa cells, RAW 264.7 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:40000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Sample-dependent, check data in validation data gallery

Product Information

66474-1-Ig targets SOD2 in WB, IHC, IF,ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Cited Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SOD2 fusion protein Ag21388
Full Name superoxide dismutase 2, mitochondrial
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC016934
Gene symbol SOD2
Gene ID (NCBI) 6648
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.

Protocols

Product Specific Protocols
WB protocol for SOD2 antibody 66474-1-IgDownload protocol
IHC protocol for SOD2 antibody 66474-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Haematologica

Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.

Authors - Nunziata Maio
ratWB,IF

Cell Death Dis

Glutathione prevents chronic oscillating glucose intake-induced β-cell dedifferentiation and failure.

Authors - Jitai Zhang
mouseWB

Front Endocrinol (Lausanne)

PMID: 31024445

Authors - Wei Ding
humanWB

J Anim Sci Biotechnol

Comparison of the protective effects of resveratrol and pterostilbene against intestinal damage and redox imbalance in weanling piglets.

Authors - Hao Zhang
pigWB

J Anim Sci Biotechnol

Resveratrol and its derivative pterostilbene ameliorate intestine injury in intrauterine growth-retarded weanling piglets by modulating redox status and gut microbiota.

Authors - Yanan Chen
mouseWB

Mol Med Rep

CART mitigates oxidative stress and DNA damage in memory deficits of APP/PS1 mice via upregulating β‑amyloid metabolism‑associated enzymes.

Authors - Hui Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Iru (Verified Customer) (07-30-2020)

This antibody works very well at 1:2000 dilution with O/N incubation.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Fallopian tube epithelial cells
SOD2 Antibody Western Blot, validation (1:2000 dilution) in Fallopian tube epithelial cells (Cat no:66474-1-Ig)