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SOD2 Monoclonal antibody
SOD2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig and More (1)
Applications
WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
3A6C2
Cat no : 66474-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | U2OS cells, HeLa cells, ROS1728 cells, RAW 264.7 cells, pig brain tissue, rat brain tissue, mouse brain tissue |
Positive IHC detected in | human liver cancer tissue, human liver tissue, human colon cancer tissue, human pancreas cancer tissue, mouse liver tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HUVEC cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 41 publications below |
IHC | See 4 publications below |
IF | See 6 publications below |
CoIP | See 1 publications below |
Product Information
66474-1-Ig targets SOD2 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat, pig, goat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | SOD2 fusion protein Ag21388 |
Full Name | superoxide dismutase 2, mitochondrial |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 25 kDa |
GenBank Accession Number | BC016934 |
Gene Symbol | SOD2 |
Gene ID (NCBI) | 6648 |
RRID | AB_2881840 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for SOD2 antibody 66474-1-Ig | Download protocol |
IHC protocol for SOD2 antibody 66474-1-Ig | Download protocol |
IF protocol for SOD2 antibody 66474-1-Ig | Download protocol |
FC protocol for SOD2 antibody 66474-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Control Release A cerium oxide loaded glycol chitosan nano-system for the treatment of dry eye disease. | ||
Stem Cell Res Ther Apoptotic vesicles resist oxidative damage in noise-induced hearing loss through activation of FOXO3a-SOD2 pathway | ||
Oxid Med Cell Longev HO-1 Contributes to Luteolin-Triggered Ferroptosis in Clear Cell Renal Cell Carcinoma via Increasing the Labile Iron Pool and Promoting Lipid Peroxidation. | ||
Mol Ther Nucleic Acids Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis | ||
J Anim Sci Biotechnol Resveratrol and its derivative pterostilbene ameliorate intestine injury in intrauterine growth-retarded weanling piglets by modulating redox status and gut microbiota. | ||
J Anim Sci Biotechnol Comparison of the protective effects of resveratrol and pterostilbene against intestinal damage and redox imbalance in weanling piglets. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Iru (Verified Customer) (07-30-2020) | This antibody works very well at 1:2000 dilution with O/N incubation.
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