|Positive WB detected in||ROS1728 cells, HeLa cells, RAW 264.7 cells, pig brain tissue, rat brain tissue, mouse brain tissue|
|Positive IHC detected in||human liver cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:10000-1:40000|
|Immunohistochemistry (IHC)||IHC : 1:2500-1:10000|
|Sample-dependent, check data in validation data gallery|
66474-1-Ig targets SOD2 in WB, IHC, IF,ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
|Tested Reactivity||Human, mouse, rat, pig|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||SOD2 fusion protein Ag21388|
|Full Name||superoxide dismutase 2, mitochondrial|
|Calculated molecular weight||25 kDa|
|Observed molecular weight||25 kDa|
|GenBank accession number||BC016934|
|Gene ID (NCBI)||6648|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.
Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.
Cell Death Dis
Glutathione prevents chronic oscillating glucose intake-induced β-cell dedifferentiation and failure.
Front Endocrinol (Lausanne)
J Anim Sci Biotechnol
Comparison of the protective effects of resveratrol and pterostilbene against intestinal damage and redox imbalance in weanling piglets.
J Anim Sci Biotechnol
Resveratrol and its derivative pterostilbene ameliorate intestine injury in intrauterine growth-retarded weanling piglets by modulating redox status and gut microbiota.
Mol Med Rep
CART mitigates oxidative stress and DNA damage in memory deficits of APP/PS1 mice via upregulating β‑amyloid metabolism‑associated enzymes.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Iru (Verified Customer) (07-30-2020)
This antibody works very well at 1:2000 dilution with O/N incubation.