CoraLite®555-conjugated SOD2 Monoclonal antibody

SOD2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3A6C2

Cat no : CL555-66474

Synonyms

IPO B, Mn SOD, MNSOD, SOD2, SODM



Tested Applications

Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66474 targets SOD2 in FC (Intra) applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SOD2 fusion protein Ag21388
Full Name superoxide dismutase 2, mitochondrial
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC016934
Gene Symbol SOD2
Gene ID (NCBI) 6648
RRIDAB_2919686
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.

Protocols

Product Specific Protocols
FC protocol for CL555 SOD2 antibody CL555-66474Download protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Anja (Verified Customer) (07-25-2022)

The antibody worked well for Flow Cytometry at a 1:100 dilution for isolated mouse cells. I would buy it again.

  • Applications: Flow Cytometry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Thymic Epithelial Cells