SOX5 Polyclonal antibody

• 

  WB analysis using 13216-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13216-1-AP (SOX5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

SOX5 Polyclonal Antibody for WB, ELISA

Cat No. 13216-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Synonyms

L SOX5, SOX5, Transcription factor SOX 5

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Tested Applications

• Positive WB detected in: A549 cells, SH-SY5Y cells, U-87 MG cells, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

13216-1-AP targets SOX5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SOX5 fusion protein Ag4092
• Full Name: SRY (sex determining region Y)-box 5
• Calculated Molecular Weight: 763 aa, 84 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC029220
• Gene Symbol: SOX5
• Gene ID (NCBI): 6660
• RRID: AB_2196089
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35711
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sex determining region Y-box protein 5 (SOX5) is a member of the SOX family, which was identified based on the conserved homology of the high-mobility group DNA-binding motif. It has been reported that SOX5 is involved in the regulation of embryonic development, and is associated with various cancer types, including prostate cancer, glioblastoma, hepatocellular carcinoma, osteosarcoma and nasopharyngeal carcinoma. SOX5 has 5 isoforms with the molecular mass of 42, 71 and 82-84 kDa. (PMID: 30854049)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Commun; VCAM1 acts in parallel with CD69 and is required for the initiation of oligodendrocyte myelination.; Authors - Yuki Miyamoto
mouse,rat	WB	Pharmacol Res; Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways.; Authors - Jiale Cai
human	WB,IHC	Cell Oncol (Dordr); Apatinib inhibits glycolysis by suppressing the VEGFR2/AKT1/SOX5/GLUT4 signaling pathway in ovarian cancer cells.; Authors - Lihua Chen ; KD Validated
human	WB	Phytomedicine; Transcriptomics, molecular docking, and cross-resistance profiling of nobiletin in cancer cells and synergistic interaction with doxorubicin upon SOX5 transfection.; Authors - Aveen N Adham ; KO Validated
human	WB	Lab Invest; Sox13 promotes hepatocellular carcinoma metastasis by transcriptionally activating Twist1.; Authors - Min Feng
mouse	IF	Mol Cell Biochem; Expression profiles of Sox transcription factors within the postnatal rodent testes.; Authors - Pauline Roumaud