|Positive WB detected in||human placenta tissue, Caco-2 cells, HT-29 cells, NCCIT cells|
|Positive IHC detected in||human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
67439-1-Ig targets SOX9 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.
|Tested Reactivity||Human, mouse|
|Cited Reactivity||human, mouse, rat, canine, rabbits|
|Host / Isotype||Mouse / IgG1|
|Immunogen||SOX9 fusion protein Ag28620|
|Full Name||SRY (sex determining region Y)-box 9|
|Calculated molecular weight||509 aa, 56 kDa|
|Observed molecular weight||56 kDa|
|GenBank accession number||BC007951|
|Gene ID (NCBI)||6662|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SOX9, also named as Transcription factor SOX-9, is a 509 amino acid protein. Sex-determining region Y (SRY)-box 9 protein (SOX9) is a member of the SOX family of transcription factors (TFs) which are developmental regulators that possess high mobility group (HMG) box DNA binding and transactivation domains. It participates in a variety of functions, such as lineage restriction and terminal diferentiation, through precise temporal and spatial expression patterns that difer between particular cell types and tissues. SOX9 gene has been implicated in different types of cancer as an oncogene; however, it also may behave as a tumor suppressor.
Mater Today Bio
Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction
Dual-dynamic-bond cross-linked injectable hydrogel of multifunction for intervertebral disc degeneration therapy
Free Radic Biol Med
Iron overload promotes intervertebral disc degeneration via inducing oxidative stress and ferroptosis in endplate chondrocytes
Decreased miR-214-3p activates NF-κB pathway and aggravates osteoarthritis progression.
Ginkgolide C slows the progression of osteoarthritis by activating Nrf2/HO-1 and blocking the NF-κB pathway
Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells.