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STAT3 Monoclonal antibody

STAT3 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

3G2D12

Cat no : 60199-1-Ig

Synonyms

Acute phase response factor, APRF, HIES, STAT3



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, RAW 264.7 cells, A549 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue, human heart tissue, human breast cancer tissue, human endometrial cancer tissue, human liver tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

60199-1-Ig targets STAT3 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, rat, zebrafish
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen STAT3 fusion protein Ag0360
Full Name signal transducer and activator of transcription 3 (acute-phase response factor)
Calculated molecular weight 770 aa, 88 kDa
Observed molecular weight 70-88 kDa
GenBank accession numberBC000627
Gene symbol STAT3
Gene ID (NCBI) 6774
RRIDAB_10913811
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a mouse monoclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.

Protocols

Product Specific Protocols
WB protocol for STAT3 antibody 60199-1-IgDownload protocol
IHC protocol for STAT3 antibody 60199-1-IgDownload protocol
IF protocol for STAT3 antibody 60199-1-IgDownload protocol
IP protocol for STAT3 antibody 60199-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

ACS Nano

Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy.

Authors - Mengshi Jiang
humanWB

J Immunother Cancer

Switch receptor T3/28 improves long-term persistence and antitumor efficacy of CAR-T cells

Authors - Songbo Zhao
mouseWB

Theranostics

Combination therapy with ropivacaine-loaded liposomes and nutrient deprivation for simultaneous cancer therapy and cancer pain relief.

Authors - Jiqian Zhang
mouseWB

Clin Transl Med

PRKAR2A-derived circular RNAs promote the malignant transformation of colitis and distinguish patients with colitis-associated colorectal cancer.

Authors - Daiwei Wan
humanWB,IF

J Invest Dermatol

Keratinocyte growth factor-2 regulates signal-transducing adaptor protein-2-mediated signal transducer and activator of transcription 3 signaling and reduces skin scar formation.

Authors - Qingde Zhou
ratWB

Cell Biol Toxicol

Protection of pancreatic β-cell by phosphocreatine through mitochondrial improvement via the regulation of dual AKT/IRS-1/GSK-3β and STAT3/Cyp-D signaling pathways

Authors - Hongyan Wang