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STAT3 Monoclonal antibody
STAT3 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 60199-1-Ig
Validation Data Gallery
|Positive WB detected in
|HeLa cells, Jurkat cells, RAW 264.7 cells, A549 cells
|Positive IP detected in
|Positive IHC detected in
|human cervical cancer tissue, human heart tissue, human breast cancer tissue, human endometrial cancer tissue, human liver tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:20000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.50 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
60199-1-Ig targets STAT3 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat samples.
|human, rat, zebrafish
|Host / Isotype
|Mouse / IgG2a
|STAT3 fusion protein Ag0360
|signal transducer and activator of transcription 3 (acute-phase response factor)
|Calculated molecular weight
|770 aa, 88 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a mouse monoclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.
|Product Specific Protocols
|WB protocol for STAT3 antibody 60199-1-Ig
|IHC protocol for STAT3 antibody 60199-1-Ig
|IF protocol for STAT3 antibody 60199-1-Ig
|IP protocol for STAT3 antibody 60199-1-Ig
|Click here to view our Standard Protocols
Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy.
J Immunother Cancer
Switch receptor T3/28 improves long-term persistence and antitumor efficacy of CAR-T cells
Combination therapy with ropivacaine-loaded liposomes and nutrient deprivation for simultaneous cancer therapy and cancer pain relief.
Clin Transl Med
PRKAR2A-derived circular RNAs promote the malignant transformation of colitis and distinguish patients with colitis-associated colorectal cancer.
J Invest Dermatol
Keratinocyte growth factor-2 regulates signal-transducing adaptor protein-2-mediated signal transducer and activator of transcription 3 signaling and reduces skin scar formation.
Cell Biol Toxicol
Protection of pancreatic β-cell by phosphocreatine through mitochondrial improvement via the regulation of dual AKT/IRS-1/GSK-3β and STAT3/Cyp-D signaling pathways