STAT3 Monoclonal antibody

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  WB analysis of HeLa using 60199-1-Ig

  WB result of STAT3 antibody (60199-1-Ig, 1:3000) with si-Control and si-STAT3 transfected HeLa cells.

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  WB analysis of HeLa using 60199-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60199-1-Ig (STAT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 60199-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60199-1-Ig (STAT3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 60199-1-Ig

  IP result of anti-STAT3 (IP:60199-1-Ig, 5ug; Detection:60199-1-Ig 1:2000) with HeLa cells lysate 1400ug.

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  IHC staining of human cervical cancer using 60199-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 60199-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 60199-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 60199-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 60199-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 60199-1-Ig(STAT3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 60199-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using STAT3 antibody (60199-1-Ig, Clone: 3G2D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

STAT3 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 60199-1-Ig

Clone No.3G2D12

Publications(87)

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

3G2D12, Acute-phase response factor, APRF, HIES, STAT 3

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human cervical cancer tissue, human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 79 publications below
• IHC: See 8 publications below
• IF: See 15 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Product Information

60199-1-Ig targets STAT3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, zebrafish, bovine
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: STAT3 fusion protein Ag0360
• Full Name: signal transducer and activator of transcription 3 (acute-phase response factor)
• Calculated Molecular Weight: 770 aa, 88 kDa
• Observed Molecular Weight: 85-88 kDa
• GenBank Accession Number: BC000627
• Gene Symbol: STAT3
• Gene ID (NCBI): 6774
• RRID: AB_10913811
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P40763
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a mouse monoclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.

Publications

Species	Application	Title
	WB,IF	ACS Nano; Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy.; Authors - Mengshi Jiang
human	WB	J Immunother Cancer; Switch receptor T3/28 improves long-term persistence and antitumor efficacy of CAR-T cells; Authors - Songbo Zhao
	WB	Theranostics; Combination therapy with ropivacaine-loaded liposomes and nutrient deprivation for simultaneous cancer therapy and cancer pain relief.; Authors - Jiqian Zhang
	CoIP	JCI Insight; PKM2-mediated collagen XVII expression is critical for wound repair; Authors - Yangdan Liu
	WB	Clin Transl Med; PRKAR2A-derived circular RNAs promote the malignant transformation of colitis and distinguish patients with colitis-associated colorectal cancer.; Authors - Daiwei Wan
	WB	Elife; Upregulated expression of ubiquitin ligase TRIM21 promotes PKM2 nuclear translocation and astrocyte activation in experimental autoimmune encephalomyelitis; Authors - Luting Yang