TGF beta 1 Polyclonal antibody

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• TGF beta 1 Polyclonal antibody (26155-1-AP)

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  WB analysis using 26155-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 26155-1-AP

  WB result of TGF beta 1 antibody (26155-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGF beta 1 transfected NIH/3T3 cells.

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  WB analysis of NIH/3T3 using 26155-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26155-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26155-1-AP (TGF beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26155-1-AP

  NIH3T3 cells were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 26155-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26155-1-AP (TGF beta 1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 26155-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26155-1-AP (TGF beta 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 26155-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using TGF beta 1 antibody (26155-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of NIH/3T3 using 26155-1-AP

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug TGF beta 1 Polyclonal antibody (26155-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

TGF beta 1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 26155-1-AP

Publications(49)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Tgfb1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, Latency-associated peptide, TGF beta1, Tgfb

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Tested Applications

• Positive WB detected in: L-929 cells, NIH/3T3 cells, RAW 264.7 cells, C6
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 31 publications below
• IHC: See 7 publications below
• IF: See 8 publications below

Product Information

26155-1-AP targets TGF beta 1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24078

  Product name: Recombinant mouse Tgfb1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 29-390 aa of NM-011577

  Sequence: GLSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESADPEPEPEADYYAKEVTRVLMVDRNNAIYEKTKDISHSIYMFFNTSDIREAVPEPPLLSRAELRLQRLKSSVEQHVELYQKYSNNSWRYLGNRLLTPTDTPEWLSFDVTGVVRQWLNQGDGIQGFRFSAHCSCDSKDNKLHVEINGISPKRRGDLGTIHDMNRPFLLLMATPLERAQHLHSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASASPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

• Full Name: transforming growth factor, beta 1
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: NM-011577
• Gene Symbol: TGF beta 1
• Gene ID (NCBI): 21803
• RRID: AB_3085844
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P04202
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TGF beta 1 is a potent cytokine capable of regulating multiple cellular processes including cell proliferation, differentiation, wound healing, and immune response. TGF beta 1 is identified in various immune cells and its activity varies in different cell types or cells at different developmental stages1. TGF beta 1 functions by binding to the TGFβ1 receptors, which subsequently activate the canonical SMAD pathway or non-canonical signaling cascades, such as the mitogen-activated protein kinase (MAPK) pathway, phosphatidylinositol-3 kinase and AKT (PI3K-AKT), and Rho family of GTPases (Rho GTPase). What is special about TGF beta 1 is its context-dependent nature that is particularly true for tumors. Full-length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB (12, 25, 45 to 65 kDa).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		ACS Nano; Inhalation of Mesenchymal-Stem-Cell-Derived Nanovesicles Delivering Catalase Enhances the Treatment for Acute Lung Injury; Authors - Linna Zhang
human	WB	Ecotoxicol Environ Saf; Ambient fine particulate matter induces cardiac fibrosis through triggering ferroptosis by heme degradation induced-iron overload; Authors - Jinjin Jiang
		Life Sci; Fecal microbiota transplantation promotes hair growth through gut microbiome and metabolic regulation; Authors - Yangyang Tian
human	IF	Biomacromolecules; Construction of Novel Polylysine/Sodium Alginate Nanocarriers Combining Chlorogenic Acid and siRNA against Keloids.; Authors - Ruiying Zhang
rat	WB,IHC	J Cereb Blood Flow Metab; Exercise-induced extracellular vesicles derived from platelet-rich plasma improved recovery after ischemic stroke; Authors - Yoshifumi Miyauchi
rat	WB	Phytomedicine; Shenling Tongqiao powder targets TGF-β1/Smad-Wnt/β-catenin axis to suppress epithelial-mesenchymal transition in chronic rhinosinusitis; Authors - Kai Liu